一阶导数分光光度法测定1%硝酸匹鲁卡品眼膏的含量

目的：改进硝酸匹鲁卡品眼膏含量测定方法。方法：采用一阶导数分光光度法测定硝酸匹鲁卡品眼膏的含量。测定波长２２２ｎｍ,△λ为２ｎｍ。结果：样品浓度在１０～６０ｍｇ／Ｌ范围内呈良好线性关系,ｒ＝０．９９９８,平均回收率为９９．８３％,ＲＳＤ％〈１．０１％。结论：方法简便、准确,可用于测定硝酸匹鲁卡品眼膏的含量。

Assaying of pilocarpine nitrate eye unguent by first derivative spectrophotometry

OBJECTIVETo find a better method to determine the concen tration of pilocarpine nitrate eye unguent.METHODSFirst derivative spectrophotymetry was used to determine the concentration of pilocarpine nitrate in pilocarpine nitrate eye unguent.The samples were determined by UV at 222nm(Δλ 2nm).RESULTSThe average recovery was 99.83%(RSD%＜1.01%).CONCLUSIONThe method was simple and accurate .It is suitable for the determination of pilocarpine nitrate in eye unguent.