| 类风湿关节炎相关的HLA—DRβ1亚型对蛋白激酶A信号的影响 |
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| 引用本文： | 栗占国,肖利群等. 类风湿关节炎相关的HLA—DRβ1亚型对蛋白激酶A信号的影响[J]. 中华内科杂志, 2001, 40(1): 19-21 |
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| 作者姓名： | 栗占国 肖利群等 |
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| 作者单位： | 栗占国（100044北京大学附属人民医院风湿免疫科）;贾汝琳（100044 北京大学附属人民医院风湿免疫科）;肖利群（Rheumatoid Arthritis Research Center, University of MichiganMedical School, USA）;Holoshitz Joseph（Rheumatoid Arthritis Research Center, University ofMichigan Medical School, USA） |
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| 基金项目： | 国家自然科学基金(39970697)及中华医学专项人才基金部分资助 |
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| 摘 要： | 目的：研究类风湿关节炎（RA）相关的HLA－DRβ1基因亚型对细胞内蛋白激酶A（PKA）信号通路的影响。方法：测定HLA－DRβ1^*0401、^*0402、^*0403、^*0404亚型转基因细胞内PKA、cAMP及腺苷环化酶（AC）的浓度及活性。结果：RA相关的HLA－DRβ1^*0410和^*0404转基因细胞的PKA活力、cAMP水平及AC活力明显低于HLA－DRβ1^*0402及^*0403转基因细胞（P值均＜0.01）。结论：RA共同表位QK／RRAA的表达可抑制PKA、cAMP和AC的活性,并可能通过干扰细胞内PKA信号通路参与RA的发病。
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| 关 键 词： | 类风湿性关节炎 HLA－DR抗原 环AMP依赖性蛋白激酶类 HLA－DRβ1亚型 |
| 修稿时间： | 2000-04-29 |
Effect of rheumatoidarthritis associated HLA-DR |
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| LI Zhanguo. Effect of rheumatoidarthritis associated HLA-DR[J]. Chinese journal of internal medicine, 2001, 40(1): 19-21 |
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| Authors: | LI Zhanguo |
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| Affiliation: | Department of Rheumatology and Immunology, People's Hospital, Beijing Medical University, Beijing 100034, China. |
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| Abstract: | OBJECTIVE: To investigate the impact of rheumatoid arthritis (RA)-associated HLA-DRbeta(1) (*)0401, (*)0402, (*)0403 and (*)0404 subtypes on protein kinase A (PKA) signaling. METHODS: To detect the activities of adenylate cyclase (AC), cAMP and PKA in transfectants expressing rheumatoid arthritis-associated HLA-DRbeta(1) subtypes and their mutants. RESULTS: The levels of AC, cAMP and PKA of HLA-DRbeta(1) (*)0401 and (*)0404 transfectants were significant lower than that of HLA-DRbeta(1) (*)0402 and (*)0403 transfectants (P < 0.01). The mutant HLA-DRbeta(1) (*)0403 transfectants expressing DRRAE or QRRAA secreted lower levels of PKA, cAMP and AC (P < 0.01). CONCLUSION: RA-associated HLA-DRbeta(1) (*)0401 and (*)0404 expression suppressed intracellular PKA signaling pathway, and may mediate abnormal intracellular signaling in rheumatoid arthritis. |
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