高压氧对异基因骨髓移植后aGVHD影响的实验研究

本研究探讨高压氧（HBO）在抗异基因骨髓移植后aGVHD和提高生存率中的作用及机制。给受致死量照射的C57BL／6受鼠注入供鼠BALB／c骨髓与脾淋巴细胞的混合液，分别给与高压氧、环孢素A、氨甲蝶呤处理。用流式细胞仪检测脾T细胞及其亚群和黏附分子CD3^＋、CD4^＋、CD8^＋、CD4^＋CD11a^＋、CD4^＋CD18^＋、CD8^＋CD11a^＋、CD8^＋CD18^＋的表达，用ELISA和RT-PCR法检测细胞因子IL-2、IL-4、IL-10和TNFα的表达。结果表明：HBO组小鼠11天的活存率明显高于异基因移植组及CsA＋MTX组；HBO和CsA＋MTX可明显降低脾组织中CD3^＋、CD4^＋、CD8^＋、CD4^＋CD11a^＋、CD4^＋CD18^＋、CD8^＋CD11a^＋、CD8^＋CD18^＋的表达（P〈0.05）；HBO组小鼠血清IL-2和TNFα的浓度及脾组织中IL-2和TNFα mRNA的含量都低于异基因骨髓移植组（P〈0.05），但高于CsA＋MTX组；HBO组小鼠脾组织中IL-4和IL-10 mRNA的含量显著高于异基因骨髓移植组和CsA＋MTX组（P〈0、05）。结论：高压氧在对抗aGVHD和提高活存率方面优于环孢素A和氨甲蝶呤，其抗aGVHD的作用机制与调节促炎／抑炎细胞因子的分泌和黏附分子的表达及抑制T淋巴细胞作用有关。

Effect of Hyperbaric Oxygen on Acute Graft-Versus-Host Disease after Allogeneic Bone Marrow Transplantation

The objective of this study was to investigate the function and mechanism of hyperbaric oxygen(HBO)in antagonizing acute graft-versus-host disease(aGVHD)and improving the rate of survival.The lethally irradiated C57BL/6 recipients were injected with bone marrow and lymphocyte of spleen from BALB/c donors and were treated kines were detccted by flow cytometry.ELISA and RT-PCR respectively.The results showed that the survival rate in HBO group was much higher than that in allogeneric bone marrow transplantation(allo-BMT)group and CsA MTX groupthe numbers of CD3 ,CD4 ,CD8 ,CD4 CD11a ,CD4 CD18 ,CD8 CD11a ,CD8 CD18 lymphotheir serum concentrations in HBO group were much lower than those in allo-BMT group but were higher than tllose in CsA MTX groupthe levels of IL-4 and IL-10 mRNA in HBO group were much higher than those in allo-BMrr group and CsA MTX group.It is concluded that HBO has more remarkable advantage in improving the rate of survival than CsA MTX,its mechanism of anti-aGVHD is tightly correlated with the transform of T cell and its subsets and the expression of adhesion molecules and cytokines.