EGFL7在人脑胶质瘤中的表达及其与微血管密度的关系

目的探讨EGFL7基因在人脑胶质瘤组织中的表达及与肿瘤微血管密度（MVD）的关系。方法收集4 5例人脑胶质瘤石蜡标本,其中低度恶性（Ⅰ～Ⅱ级）2 4例,高度恶性（Ⅲ～Ⅳ级）2 1例,应用免疫组织化学检测EGFL7蛋白的表达情况,应用八因子免疫组化标记肿瘤微血管。分析EGFL7蛋白表达与胶质瘤病理分级及MVD的关系。结果EGFL7在人脑胶质瘤中存在表达,EGFL7蛋白表达主要位于肿瘤细胞及血管内皮细胞的胞浆。其表达与肿瘤的恶性程度（t=4.3 9 9,P＆lt;0.0 1）及MVD（r=0.6 8 4,P＆lt;0.0 1）有明显的相关性;EGFL7在正常人脑组织中无表达。结论人脑胶质瘤存在EGFL7基因的表达,并且与肿瘤恶性程度及MVD具有明显相关性,提示EGFL7在人脑胶质瘤的血管生成中可能发挥重要作用。

Expression of EGFL7 in human glioma and its correlation with microvessel density of tumour

Objective To investigate the expression of epidermal growth factor-like domain 7（EGFL7） in human glioma and its correlation with microvessel density（MVD） of the tumor.Methods The expression of EGFL7 protein was deteced by immunohistochemical staining in 45 formalin-fixed,paraffin-embedded specimens of glioma,including 24 of low malignance（grade ⅠⅡ） and 21 of high malignance（grade ⅢⅣ）.MVD in all the specimens was detected by Factor Ⅷ immunohistochemical staining.The correlation between EGFL7 expression and MVD of the tumour was analyzed.Result The expression of EGFL7 could be detected in human glioma,but could not in normal brain tissue;its major locations were the plasm of tumor cells and endothelial cells.The expression correlated directly with the degree of malignancy and MVD.Conclusion EGFL7 gene can express itself in human glioma cells and endothelial cells;the level of expression directly correlated with the degree of malignancy and MVD,which suggests that EGFL7 may play a unique role in the process of glioma angiogenesis.