肺癌组织中多药耐药基因及相关蛋白表达的研究

目的:探讨P-糖蛋白(P-glycopro-tein,P-gp)、肺癌耐药蛋白(lung cancer resis-tence protein,LRP)和多药耐药相关蛋白(multidrug resistence protein,MRP)在肺癌组织中的表达及其临床意义。方法:应用SP法检测116例术前未做化疗的肺癌组织中P-gp、LRP和MRP的表达水平。结果:P-gp在腺癌组织中的阳性表达率为78.26%(36/46),小细胞癌为63.64%(7/11),鳞状细胞癌为47.46%(28/59);三者相比差异有统计学意义,P=0.018。LRP在腺癌组织中的阳性表达率为89.13%(41/46),鳞状细胞癌为44.07%(26/59),小细胞癌为27.27%(3/11);三者相比差异有统计学意义,P=0.0001;MRP在不同癌组织中的表达差异无统计学意义,P=0.4165。在腺癌、鳞状细胞癌和小细胞癌组织中同时有两种或两种以上耐药基因产物表达阳性率分别为89.13%(41/46)、49.15%(29/59)和27.28%(3/11),三种类型间比较差异有统计学意义,P=0.0001。结论:不同组织学类型的肺癌存在不同程度的耐药性,检测P-gp、LRP和MRP的协同表达对于指导临床化疗方案的实施有重要意义。

Expressions of multidrug resistance markers P-gp, MRP, LRP in lung cancer tissues

OBJECTIVE:To investigate the expression levels and clinical significance of multidrug resistance(MDR)markers(P-gp,LRP and MRP)in lung cancer tissues.METHODS:The expressions of lung cancer resistance protein(LRP),multidrug resistance protein(MRP)and P-glycoprotein(P-gp)of MDR markers were detected in 116 cases of lung cancer tissues by the streptavidin-biotin-peroxidase(SP)immunohistochemistry technique.RESULTS:There was an overexpression of P-gp in different types of lung cancer tissues and the expression of adenocarcinoma(78.26%),small cell carcinoma(63.64%)and squamous cell carcinoma(47.46%)were significantly different,P=0.018.The expression of LRP in adenocarcinoma(89.13%)was higher than that in squamous carcinoma(44.07%)and that in small cell carcinoma of lung(27.27%),P=0.000 1.No significant difference was observed in the expression of MRP among the different types of lung cancer,P=0.416 5.The expression rates of two kinds or more of MDR markers in the adenocarcinoma,squamous carcinoma and small cell carcinoma were 89.13%,49.15% and 27.28% respectively,P=0.000 1.CONCLUSIONS:There are different degrees of overexpressions of multidrug resistant markers in the different types of lung cancer.Combined detection of P-gp,LRP and MRP may be useful for guiding the clinical chemotherapy of lung cancer.