| 人ω干扰素克隆、表达及体外抑制冠状病毒昆徐株作用的研究 |
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| 引用本文： | 杨子峰,朱宇同,张奉学,杨瑞仪,刘妮,赵昉.人ω干扰素克隆、表达及体外抑制冠状病毒昆徐株作用的研究[J].国际呼吸杂志,2008,28(6). |
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| 作者姓名： | 杨子峰 朱宇同 张奉学 杨瑞仪 刘妮 赵昉 |
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| 作者单位： | 1. 广州呼吸疾病国家重点实验室,广州医学院第一附属医院,510230
2. 广州中医药大学热带医学研究所,510405 |
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| 摘 要： | 目的 制备人ω干扰素(hIFN-ω)并初步研究其体外抗普通冠状病毒活性.方法 制备hIFN-ω cDNA,并在大肠杆菌BL-21中高效表达、纯化后,测定了hIFN一ω的抗滤泡口膜炎病毒(VSV)和冠状病毒昆徐株活性.结果 成功地获得了hIFN-ω GST融合蛋白,利用WISH-VSV系统检测hIFN-ω的抗病毒活力为7.81×104 kU/L;与基因工程α干扰素(IFN-a2b)的比较发现,hIFN-ω可以明显抑制冠状病毒昆徐株的活性,而IFN-α2b未见此活性.结论 hIFN-ω具有抑制冠状病毒昆徐株的活性,有独特的抗呼吸道病毒作用;不同的干扰素亚型对不同的病毒敏感性有所差异.
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| 关 键 词： | 干扰素类 抗病毒药 冠状病毒 |
Cloning, expression of human interferon-ω and its anti-coronavirus Kunxu strain effect in vivo |
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| YANG Zi-feng,ZHU Yu-tong,ZHANG Feng-xue,YANG Rui-yi,LIU Ni,ZHAO Fang.Cloning, expression of human interferon-ω and its anti-coronavirus Kunxu strain effect in vivo[J].International Journal of Respiration,2008,28(6). |
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| Authors: | YANG Zi-feng ZHU Yu-tong ZHANG Feng-xue YANG Rui-yi LIU Ni ZHAO Fang |
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| Abstract: | Objective To obtain the human interferon-ω and observe its anti-coronavirus activities.Methods The cDNA of human interferion-ω was obtained,and the IFN-ω fusion protein was expressed and purified in Escherichla colt(E.eoli)BL-21.The anti-virus activities of human interferon-ω were accessed bv using WISH-VSV system,and then compared with those of IFN-a2b by using coronavirus(Kunxu strain).Results GST-IFN-ω fusion protein was obtained successfully.And the anti-virus activity of IFN-ω was 7.81×104 kU/L,which was accessed by WISH-VSV system.Compared with IFN-α2b,IFN-ω inhibited cytopathic effect of coronavirus(Kunxu strain),and IFN-α2b had no such effect.Conclusions IFN-ωcould inhibit coronavirus，indicating that in may have special way on anti-respirovirus.Also sensibilities were different among interferon subtypes. |
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| Keywords: | Interferon Antiviral medicine Coronavirus |
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