病毒性心肌炎小鼠心肌细胞凋亡及心肌病变的电子显微镜观察

目的研究病毒性心肌炎(VMC)小鼠心肌超微结构改变及细胞凋亡的形态学变化。方法本实验在接种柯萨奇病毒B3(CVB3)建立VMC动物模型的基础上,用光学显微镜及电子显微镜观察心肌细胞的病变及心肌细胞凋亡。结果研究发现实验组小鼠在接种病毒5d后光镜或电镜下可见心肌病变及炎细胞浸润,7-9天病变达高峰,35天时病变基本恢复。VMC小鼠在接种病毒后7-9天,电镜下可见心肌细胞呈凋亡样改变,并可见凋亡小体。结论实验组小鼠在接种CVB3后可引起VMC,VMC中存在异常的心肌细胞凋亡现象。

Electron Microscope Observation of Cardiomyocyte Apoptosis and Myocardial Change in Viral Myocarditis

Objective To study ultrastructure changes of myocardium and morphic change of cardiomyocyte apoptosis in mice with viral myocarditis(VMC).Methods Our experiment established animal model of VMC by the way of coxsackie virus B3(CVB3) inoculation,then we studied the changes and apoptosis of cardiomyocyte by ways of microscope and electron microscope.Results The myocardial changes and inflammatory cell infiltration were found by ways of microscope and electron microscope from 5 days after virus inoculation in experimental mice,the peak changes were at 7-9 days,and were almost recoverd at 35 days.Apoptotic changes and apoptotic bodies were found by eletron microscope at 7-9 days after virus inoculation in mice with VMC.Conclusions VMC can be caused in mice after CVB3 inoculation,abnormal cardiomyocyte apoptosis can be found in VMC.