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Immunofluorescent detection of anti-cytoskeleton antibodies using vinblastine-treated mononuclear cells |
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| Authors: | D Zauli C Crespi F B Bianchi E Pisi |
| Affiliation: | Istituto di Patologia Speciale Medica I, University of Bologna, Bologna, Italy |
| Abstract: | A reliable and reproducible immunofluorescence method is described for the detection of anti-cytoskeleton antibodies in human sera, based on the use of vinblastine-treated peripheral blood mononuclear cells as substrate. Three immunofluorescence patterns associated with antibodies to microfilaments, intermediate filaments and microtubules are readily identified. |
| Keywords: | anti-cytoskeleton antibodies intermediate filaments microfilaments microtubules mononuclear cells IMF intermediate filaments MF microfilaments MT microtubules MC mononuclear cells PBS phosphate-buffered saline IFL immunofluorescence |
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