Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis |
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| Authors: | Bens Corina C P M Voss Andreas Klaassen Corné H W |
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| Institution: | Department of Medical Microbiology and Infectious Diseases, Regional Public Health Laboratory, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands. |
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| Abstract: | Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG. |
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