A modified photometric quantification of skin surface lipids sampled using the cigarette paper method |
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Authors: | S Bolanča-Bumber A Basta-Juzbasić A Kansky T Gross |
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Institution: | (1) Department of Dermatology, Clinical Center Zagreb, Yugoslavia;(2) Institute of Gerontology, Clinical Center Ljubljana, Yugoslavia |
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Abstract: | Summary In severe cases of acne conglobata the use of substances which considerably reduce the sebum excretion rate, such as 13-cis-retinoic acid, is reasonable. To monitor the effect of such preparation a noninvasive and accurate method for measuring skin surface lipids (SSLs) is required. A new modified photometric quantification of SSLs sampled using the cigarette paper method based on reaction with phosphovanilic reagent was introduced. Our overall results indicate that a 3-h SSLs assessment measured photometrically is as accurate as the gravimetric technique; it is reproducible in situations with low and high levels of SSLs. |
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Keywords: | Sebum excretion Skin surface lipids Cigarette paper method |
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