| 大青叶中靛玉红的提取分离工艺研究 |
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| 引用本文： | 谢国祥,张立国,邱明丰,贾伟. 大青叶中靛玉红的提取分离工艺研究[J]. 中成药, 2006, 28(6): 791-793 |
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| 作者姓名： | 谢国祥 张立国 邱明丰 贾伟 |
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| 作者单位： | 1. 上海交通大学,药学院,上海,200240
2. 华东理工大学,化学与制药学院,上海,200237 |
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| 摘 要： | 目的:采用近红外光谱(NIR)技术对大青叶中的有效成分靛玉红的提取分离工艺进行研究。方法:采用氧化铝柱色谱法分离靛玉红,氯仿-醋酸乙酯作为洗脱剂,洗脱液用甲醇-丙酮重结晶。结果:此工艺所得靛玉红纯度为97.78%,且样品与对照品纯度相接近,NMR和IR图谱相同。结论:该法简便易行,安全、成本低、方便,实验结果良好。
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| 关 键 词： | 大青叶 靛玉红 柱色谱法 近红外光谱 |
| 文章编号： | 1001-1528（2006）06-0791-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-30 |
Extraction and seperation of indirubin in Folium Isatidis |
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| XIE Guo-xiang,ZHANG Li-guo,QIU Ming-feng,JIA Wei. Extraction and seperation of indirubin in Folium Isatidis[J]. Chinese Traditional Patent Medicine, 2006, 28(6): 791-793 |
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| Authors: | XIE Guo-xiang ZHANG Li-guo QIU Ming-feng JIA Wei |
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| Affiliation: | 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China ; 2. School of Chemistry and Pharmaceutical, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | AIM: To study the suitable conditions of the extraction-separation process of the indirubin in Folium Isatidis with NIR. METHODS: The indirubin was chromatographed on an aluminium oxide column and chloroform-ethyl acetate as eluting agent,eluate was recrystalized by methanol-acetone. RESULTS: The indirubin extracted by this method was of high purity (97.78%).The sample purity was similar to reference substance one((98.01%)).The spectra of NMR and IR are the same. CONCLUSION: This method is proved to be fast,safe,lower cost,simple and has good results. |
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| Keywords: | Folium Isatidis indirubin column chromatograph NIR |
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