| 高效液相色谱法测定人血浆中异烟肼和吡嗪酰胺的含量 |
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| 引用本文： | 杨正管,王庆彪,刘莹.高效液相色谱法测定人血浆中异烟肼和吡嗪酰胺的含量[J].中国药科大学学报,2000,31(2):27-29. |
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| 作者姓名： | 杨正管 王庆彪 刘莹 |
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| 作者单位： | 南京军区福州总医院药理科!福州350025 |
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| 摘 要： | 建立了同时测定人血浆中异烟肼和吡嗪酰胺的反相高效液相色谱法。色谱柱Ｌｉｃｈｒｏｓｐｈｅｒ １００ＲＰ－Ｃ８（５μｍ）流动相为乙腈（Ａ）：０．０５ｍｏｌ／Ｌ磷酸二氢钾（Ｂ）（每５００ｍｌ中加入三乙胺约２００μｍｌ调ｐＨ５．６），检测波长２７０ｎｍ。线性范围分别为０．１￣１０μｇ／ｍｌ和１￣４０μｇ／ｍｌ，方法回收率分别为大于９０％和８２％，日内，日间差皆小于１０％。
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| 关 键 词： | 异烟肼 吡嗪酰胺 高效液相色谱 血药浓度 |
Determination of Isoniazid and Pyrazinamide in Plasma by HPLC |
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| YANG Zheng Guan,WANG Qing Biao,LIU Ying.Determination of Isoniazid and Pyrazinamide in Plasma by HPLC[J].Journal of China Pharmaceutical University,2000,31(2):27-29. |
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| Authors: | YANG Zheng Guan WANG Qing Biao LIU Ying |
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| Institution: | YANG Zheng Guan,WANG Qing Biao,LIU Ying Department of Clinical Pharmacology of Dongfang Hospital,Fuzhou 350025 |
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| Abstract: | A HPLC method was established to determine the plasma concentration of isoniazid and pyrazinamide. Lichrospher 100 RP C 8 was used as a statephase. A linear gradient delution was used from a binary system consisting of solvent A (acetonitrile ) and solvent B (0.05 mol/L KH 2PO 4).Quantitation of isoniazid and pyrazinamide was achieved with a high precision and a low inter sample variation. |
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| Keywords: | Isoniazid Pyrazinamide HPLC Plasma concentration Determination of content |
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