| Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions |
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| 引用本文： | 陈善娟,李少华,吴艳,刘志香,李家文. Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions[J]. 华中科技大学学报(医学英德文版), 2008, 28(4): 476-479. DOI: 10.1007/s11596-008-0423-z |
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| 作者姓名： | 陈善娟 李少华 吴艳 刘志香 李家文 |
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| 作者单位： | Department of Dermatology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Ophthalmology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology |
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| 基金项目： | This project was supported by a grant from the Science Research Foundation of Health Department of Hubei Province （No. JXIB048） and Janssen Research Foundation. |
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| 摘 要： | To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 （IL-2）/Th2 （IL-4, IL-10, TGF-β1） cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-β1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-β1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans （C. albicans）, and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice.
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| 关 键 词： | 白念珠菌 阴道冲洗 鼠科 Th1/Th2 |
| 收稿时间： | 2009-02-04 |
Local expression of vaginal Th1 and Th2 cytokines in murine vaginal candidiasis under different immunity conditions |
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| Shanjuan Chen,Shaohua Li,Yan Wu,Zhixiang Liu,Jiawen Li. Local expression of vaginal Th1 and Th2 cytokines in murine vaginal candidiasis under different immunity conditions[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(4): 476-479. DOI: 10.1007/s11596-008-0423-z |
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| Authors: | Shanjuan Chen Shaohua Li Yan Wu Zhixiang Liu Jiawen Li |
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| Affiliation: | 1. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 (IL-2)/Th2 (IL-4, IL-10, TGF-beta1) cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-beta1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-beta1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans (C. albicans), and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice. |
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| Keywords: | Candida albicans vaginal lavage murine model immuno-suppression Th1/Th2 cytokinese |
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