睡眠剥夺对大鼠血清MBP及皮质醇含量的影响

目的　测定大鼠睡眠剥夺 ( sleep deprivation,SD)后血清髓鞘碱性蛋白 ( myelin basic protein,MBP)和皮质醇水平变化 ,观察 SD对大脑的损害情况。方法　采用小平台水环境法建立 SD模型 ,以大平台组 ( TC)和正常笼养组 ( CC)作为对照组 ,采用酶联免疫吸附法测定 MBP水平 ,并用双抗体放射免疫法测定皮质醇水平。观察大鼠经 1、3、5 d SD后上述指标变化。结果　与 CC组比较 ,SD 1、3、5 d后血清皮质醇水平均增高 ,与 TC组比较 ,SD3、5 d血清皮质醇水平增高 ,差异有显著性 ;SD5 d后血清 MBP水平增高 ,SD1、3 d水平差异无显著性。TC组与 CC组比较 ,血清皮质醇水平增高而 MBP水平差异无显著性。结论　长时间 SD可引起脑器质性损害。

Effects of Sleep Deprivation on the Levels of MBP and Cortisol in Serum of Rats

Objective To investigate effects of sleep deprivation (SD) on the levels of MBP and cortisol in serum of rats. Methods Paradoxical sleep deprivation was induced in male Sprague-Dawley rats by housing them on small platforms over water. Controls were housed either in normal cages (CC) or in tanks with large platforms(TC).After SD for different lengths of time,seurm levels of cortisol of all the groups were measured by double antibodies radioimmune assay and myelin basic protein(MBP)were measured using enzyme linked immuno-absorbent assay. Results Compared with CC and TC groups, seurm levels of cortisol of SD 1 d, 3 d, 5 d groups, and seurm levels of MBP of SD 5 d were increased significantily. While seurm levels of cortisol of TC group were increased as compared with normals. Conclusions There are brain damages in rat after prolonged SD.