| 槲寄生多糖的提取分离和含量测定 |
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| 引用本文： | 王俊,朱一凡.槲寄生多糖的提取分离和含量测定[J].中国中药杂志,2007,32(22):2387-2390. |
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| 作者姓名： | 王俊 朱一凡 |
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| 作者单位： | 江苏科技大学,生物与环境工程学院,江苏,镇江,212018 |
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| 基金项目： | 江苏省高校自然科学基金 |
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| 摘 要： | 目的：对国产槲寄生中的有效成分多糖进行提取分离和含量测定。方法：采用热水提取法提取槲寄生多糖,超滤膜分离-离子交换色谱法分离纯化多糖组分；槲寄生多糖经苯酚-硫酸显色后,在490 nm处进行比色测定。结果：槲寄生药材、槲寄生粗多糖CVPS-Ⅲ和槲寄生精制多糖CVPS-Ⅲ-C中的多糖含量分别为4.93%(RSD 1.04%,n=3),43.28%(RSD 1.39%,n=3)和69.55%(RSD 1.62%,n=3),平均回收率为96.07%(RSD 2.54%,n=5)。结论：该法简便、快速、准确、灵敏度好。
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| 关 键 词： | 槲寄生 多糖 提取分离 含量测定 |
| 文章编号： | 1001-5302（2007）22-2387-04 |
| 收稿时间： | 2006-12-28 |
| 修稿时间： | 2006年12月28 |
Extraction and content determination of polysaccharides in Viscum coloratum |
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| WANG Jun; ZHU Yi-fan.Extraction and content determination of polysaccharides in Viscum coloratum[J].China Journal of Chinese Materia Medica,2007,32(22):2387-2390. |
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| Authors: | WANG Jun; ZHU Yi-fan |
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| Institution: | College of Biology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China |
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| Abstract: | OBJECTIVE: To establish a method for extraction and content determination of polysaccharide in Viscum coloratum. METHOD: Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent. RESULT: The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5). CONCLUSION: The method was simple, rapid, and accurate. |
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| Keywords: | Viscum coloratum polysaccharide extraction and separation content determination |
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