良恶性胶质瘤的核仁组织区定量研究

使用胶质银染色技术对80例胶质瘤进行核仁组织区(AgNORs)定量研究.其中星形细胞瘤49例(星形细胞瘤Ⅰ级13例,Ⅱ级11例,Ⅲ级25例),室管膜瘤16例(良性室管膜瘤6例,恶性室管膜瘤10例),髓母细胞瘤15例.观察结果表明:星形细胞瘤Ⅰ级和Ⅱ级之间平均每核含Ag—NORs颗粒数相差显著(P<0.05),星形细胞瘤Ⅰ级和Ⅲ级、Ⅱ级和Ⅲ级及良、恶性室管膜瘤之间平均每核AgNORs颗粒数均相差非常显著(P<0.01).提示AgNORs定量研究有助于良、恶性胶质瘤的鉴别,对肿瘤的分级有一定参考价值.

Quantitative study of AgNORs in benign and malignant neuroglial tumors

Using silver colloid staining technique, nucleolar organizer region-associated proteins ( AgNORs ) were studied with quantitative methods in paraffin sectiom of 80 neuroglial tumors, including 49 astrocytomas ( 13 in grade I , 11 grade II and 25 grade III ) , 16 ependymomas ( 6 benign, 10 malignant) and 15 medullobastomas. The results showed that there is a significant difference in the mean number of AgNORs per nucleus in astrocytoma between grade land I ( P<0.05) , grade I and grade III, grade II and grade III and in ependymomas between benign and malignant ( P<0.01 ) . It was suggested that this simple technique may be diagnostic-ally helpful in differentiating benign and malignant neuroglial tumors and in grading tumors.