| SUMO-1基因沉默对肝癌细胞株SMMC-7721bcl-2及c-myc基因表达的影响 |
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| 引用本文： | 袁丽华,郭武华,肖志华,张吉翔.SUMO-1基因沉默对肝癌细胞株SMMC-7721bcl-2及c-myc基因表达的影响[J].重庆医学,2010,39(19). |
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| 作者姓名： | 袁丽华 郭武华 肖志华 张吉翔 |
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| 作者单位： | 江西省分子生物重点实验室/南昌大学第二附属医院消化科,江西南昌,330006;江西省分子生物重点实验室/南昌大学第二附属医院肿瘤科,江西南昌,330006 |
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| 摘 要： | 目的 探讨SUMO-1基因沉默后对肝癌细胞株SMMC-7721 bcl-2及c-myc表达的影响.方法 将人工合成的针对人SUMO-1基因的siRNA片段转染人肝癌细胞株SMMC-7721,采用RT-PCR、Western blot方法检测siRNA沉默SUMO-1基因的效果及SUMO-1基因沉默后对bcl-2及c-myc基因表达的影响.结果 人工合成的针对SUMO-1基因的siRNA片段能显著地抑制SMMC-7721 SUMO-1基因的表达,24、48、72 h沉默率分别为9.80%、73.43%、46.56%.SUMO-1基因表达下调后SMMC-7721 细胞内bcl-2及c-myc基因表达也明显下调,差异有统计学意义.结论 siRNA是一种理想的沉默SMMC-7721 SUMO-1基因表达的方法,SUMO-1基因沉默后SMMC-7721细胞内的bcl-2及c-myc基因表达均明显下调,说明SUMO-1基因控制癌基因bcl-2及c-myc的表达.
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| 关 键 词： | SUMO-1 siRNA 肝癌 bcl-2 c-myc |
SUMO-1 controls the expression of bcl-2 and c-myc in HCC cell line SMMC-7721 |
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| YUAN Li-hua,GUO Wu-hua,XIAO Zhi-hua,et al..SUMO-1 controls the expression of bcl-2 and c-myc in HCC cell line SMMC-7721[J].Chongqing Medical Journal,2010,39(19). |
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| Authors: | YUAN Li-hua GUO Wu-hua XIAO Zhi-hua |
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| Institution: | YUAN Li-hua1,GUO Wu-hua2,XIAO Zhi-hua1,et al.(1.Department of Gastroenterology,2.Department of Oncology,the Second Affiliated Hospital of Nanchang University/Jiangxi Provincial Key Laboratory of Molecular Medicine,Nanchang,Jiangxi 330006,China) |
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| Abstract: | Objective To study the efficiency of silencing SUMO-1 induced by siRNA in hepatocellular carcinoma cell line SMMC-7721 and to explore the inhibition of bcl-2 and c-myc expression after being silenced SUMO-1 gene in vitro.Methods The SUMO-1 siRNA was transfected into SMMC-7721 by means of LipofectamineTM 2000.The silencing efficiency of SUMO-1 was examined by RT-PCR and Western blot.The expressions of bcl-2 and c-myc were examined by RT-PCR and Western blot.Results The siRNA could significantly silence the e... |
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| Keywords: | SUMO-1 siRNA bcl-2 c-myc |
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