| 抗T-2毒素单克隆抗体的制备及鉴定 |
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| 引用本文： | 王雄,齐红莉,吴继宗,陈冰君,果旗,王艳斐,荣钊. 抗T-2毒素单克隆抗体的制备及鉴定[J]. 中国卫生检验杂志, 2010, 0(2) |
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| 作者姓名： | 王雄 齐红莉 吴继宗 陈冰君 果旗 王艳斐 荣钊 |
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| 作者单位： | 北京中检维康技术有限公司;中国原子能科学研究院;天津农学院水产生态及养殖重点实验室; |
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| 摘 要： | 目的:制备并鉴定抗T-2毒素单克隆抗体杂交瘤细胞株,以期为T-2毒素的快速检测提供有力的抗体工具。方法:采用琥珀酸酐法将T-2毒素活化为T-2HS,T-2HS在DCC与NHS作用下与牛血清白蛋白(BSA)或卵清白蛋白(OVA)偶联合成完全抗原。以T-2HS-BSA为抗原,免疫BALB/c小鼠,用ELISA筛选、建立分泌抗T-2毒素抗体的杂交瘤细胞株。测定单抗免疫球蛋白亚类及单抗效价,用间接竞争法检测单克隆抗体细胞株的特异性。结果:建立了1株稳定分泌抗T-2毒素抗体的杂交瘤细胞株T-1B3,该细胞株所分泌的单隆抗体Ig亚类为IgG1,ELISA检测结果表明抗T-2毒素抗体可以与T-2毒素发生特异性反应,工作浓度为1:6.5×104,IC50为3.6 ng/ml。该抗体与HT-2毒素的交叉反应为65.9%,与黄曲霉毒素无交叉。结论:制备出能分泌具有较高特异性和亲和力的抗T-2毒素单克隆抗体的杂交瘤细胞株。
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| 关 键 词： | T-2毒素 杂交瘤细胞 单克隆抗体 ELISA |
Preparation and characterization of a monoclonal antibody against T-2 toxin |
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| WANG Xiong,,QI Hong-li,WU Ji-zong,CHEN Bing-jun,GUO Qi,WANG Yan-fei,RONG Zhao. Preparation and characterization of a monoclonal antibody against T-2 toxin[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(2) |
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| Authors: | WANG Xiong QI Hong-li WU Ji-zong CHEN Bing-jun GUO Qi WANG Yan-fei RONG Zhao |
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| Affiliation: | WANG Xiong1,3,QI Hong-li2,WU Ji-zong3,CHEN Bing-jun1,GUO Qi1,WANG Yan-fei1,RONG Zhao1(1.Clover Technology Group Inc.,Beijing 100044,China,2.Tianjin Agricultural University,Tianjin 300384,3.Institute of Atomic Energy,Beijing 102413,China) |
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| Abstract: | Objective:To establish a hybridoma cell line secreting monoclonal antibody(McAb) against T-2 toxin to a rapid assay.Methods:T-2 toxin was converted to T-2-hemisuccinate(T-2HS) and conjugated to BSA and OVA.Hybridoma cell line secreting monoclonal antibody against T-2 toxin was produced by using B cell hybridoma technique,The Ig subtype and titer of the McAbs against T-2 toxin were assayed with ELISA.Results:One hybridoma cell line secreting monoclonal antibodies against T-2 toxin(T-1B3),it was obtained by f... |
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| Keywords: | T-2 toxin Hybridoma Monoclonal antibody ELISA |
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