In vitro fertilization and gamete micromanipulation in the lowland gorilla |
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| Authors: | S. E. Lanzendorf W. J. Holmgren N. Schaffer H. Hatasaka A. C. Wentz R. S. Jeyendran |
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| Affiliation: | (1) Section of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Prentice Women's Hospital, Northwestern University Medical School, Suite 1504, 333 East Superior Street, 60611 Chicago, Illinois, USA;(2) Present address: Jones Institute Research Laboratories, Eastern Virginia Medical School, 23510 Norfolk, Virginia, USA;(3) Lincoln Park Zoo, 60614 Chicago, Illinois, USA;(4) Present address: Department of Obstetrics and Gynecology, University of Utah, 84132 Salt Lake City, Utah, USA |
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| Abstract: | Purpose Our purpose was to utilize current reproductive technologies to treat an infertile female lowland gorilla.Results Following pituitary down-regulation with a gonadotropin releasing hormone agonist and follicular stimulation with human menopausal gonadotropins, transrectal ultrasound-guided aspiration of ovarian follicles yielded nine oocytes. Following failed fertilization with both epididymal and electroejaculated sperm, zona dissection and sperm injection were performed and produced one embryo, which was cryopreserved. Immature oocytes were obtained from ovarian sections and were either cultured in vitro or cryopreserved.Conclusions This report demonstrates that in vitro fertilization techniques, including pituitary suppression, ultrasound-guided aspiration, micromanipulation, and cryopreservation, can be used to assist reproduction in the lowland gorilla. |
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| Keywords: | in vitro fertilization lowland gorilla micromanipulation ovarian hyperstimulation |
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