| NF-κB活化在IL-1β诱导的小鼠肾小球系膜细胞表达IL-6中的作用 |
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| 引用本文： | 许韩师,叶任高,等.NF-κB活化在IL-1β诱导的小鼠肾小球系膜细胞表达IL-6中的作用[J].中国病理生理杂志,2001,17(5):428-430. |
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| 作者姓名： | 许韩师 叶任高 |
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| 作者单位： | 中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广东广州510080 |
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| 基金项目： | 中山医科大学211工程项目基金资助(No.98150) |
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| 摘 要： | 目的:研究NF-κB在rhIL-1β刺激引起的体外培养的鼠肾小球系膜细胞表达IL-6中的作用。方法:NF-κΒ活性检测采用电泳迁移率改变法(EMSA),IL-6mRNA表达采用逆转录/聚合酶链反应(RT/PR)检测,培养上清IL-6蛋白含量采用ELISA检测。结果:rhIL-1β刺激肾小球系膜细胞时,在上调IL-6蛋白和基因表达的同时亦激活NF-κB,而且这种上调作用可被NF-κB特异性抑制剂PDTC所阻抑。结论:IL-1β诱导鼠肾小球系膜细胞表达IL-6是通过NF-κB调控,NF-κB可能参与肾小球肾炎的免疫炎症反应。
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| 关 键 词： | 白细胞介素1 白细胞介素6 NF-κB 肾小球 小鼠 |
| 文章编号： | 1000-4718(2001)05-0428-03 |
| 收稿时间： | 2000-01-06 |
| 修稿时间： | 2000年1月6日 |
Role of nuclear factor |
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| XU Han-shi,YE Ren-gao,YANG Qiong-qiong,SUN Lin,YANG Nian-sheng,LI You-ji,ZENG Li-xia.Role of nuclear factor[J].Chinese Journal of Pathophysiology,2001,17(5):428-430. |
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| Authors: | XU Han-shi YE Ren-gao YANG Qiong-qiong SUN Lin YANG Nian-sheng LI You-ji ZENG Li-xia |
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| Institution: | Department of Nephrology, The First Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China |
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| Abstract: | AIM:To investigate the regulatory role of nuclear factor κB (NF-κB) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1β.METHODS:Activation of NF-κB was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS:rhIL-1β could rapidly stimulate the activation of NF-κB in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-κB, could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1β.CONCLUSION:IL-6 expression induced by IL-1β may be regulated by NF-κB in MC, NF-κB may modulate the immune-inflammatory reaction in glomerular disease. |
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| Keywords: | Interleukin-1 Interleukin-6 NF-Kappa B Kidney glomerulus Mice |
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