Impact of Dentin Substrate Modification with Chitosan-Hydroxyapatite Precursor Nanocomplexes on Sealer Penetration and Tensile Strength |
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| Institution: | 1. Kishen Lab, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada;2. School and Hospital of Stomatology, Tianjin Medical University, Tianjin, Shi, China;3. Department of Dentistry, Mount Sinai Health System, Mount Sinai Hospital, Toronto, Ontario, Canada;1. Department of Orthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China;2. Department of Pathology, School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama;3. Oral Biomedical Engineering Laboratory, Shanghai Stomatological Hospital, Fudan University, Shanghai, China;1. Unit of Endodontology, Division of Restorative Dental Science, University College London Eastman Dental Institute, London, United Kingdom;2. Division of Biomaterials and Tissue Engineering, University College London Eastman Dental Institute, London, United Kingdom;3. The Discoveries Centre for Regenerative and Precision Medicine, University College London, London, United Kingdom;4. Department of Nanobiomedical Science and BK21 Plus NBM, Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea;6. University College London Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea;1. Department of Endodontics, São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil;2. Department of Endodontics, School of Dentistry, Pontifical Catholic University of Campinas, Campinas, São Paulo, Brazil;1. Department of Stomatology, Shenzhen Nanshan People''s Hospital and the Affiliated Shenzhen Sixth Hospital of Guangdong Medical University, Guangdong Province, China;2. Central Laboratory, Shenzhen Nanshan People''s Hospital and the Affiliated Shenzhen Sixth Hospital of Guangdong Medical University, Guangdong Province, China;3. School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, China;4. Department of Restorative Dentistry, University of Washington School of Dentistry, Seattle, Washington;6. Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China;5. Division of Oral Health Sciences, Medical and Dental Sciences Track, Department of Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan;1. Guangdong Province Key Laboratory of Stomatology, Guangzhou, Guangdong, China;2. Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China;3. Zhujiang New Town Dental Clinic, Guanghua School and Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China |
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| Abstract: | IntroductionThe purpose of this study was to evaluate the effect of dentin conditioning with chitosan-hydroxyapatite precursor (C-HA) nanocomplexes on the depth of tricalcium silicate sealer penetration into dentinal tubules and ultimate tensile strength (UTS).Methodssurface charge and size distribution for C-HA nanocomplex formulation was evaluated followed by bioactivity assessment of standardized films of C-HA nanocomplexes (n = 15) incubated in simulated body fluid. Mineralization potential was assessed with X-ray diffraction and Fourier-transform infrared spectroscopy, whereas scanning electron microscopy was used for ultrastructural evaluation. Static water contact angles and UTS were measured on dentin discs (n = 2/group) and dentin beams (n = 10/group) treated with/without sodium hypochlorite/EDTA and C-HA nanocomplex conditioning. In phase 2, the depth of sealer penetration after C-HA nanocomplex conditioning was evaluated using fluorescent imaging (n = 12/group). The percent area penetration and mean/maximum penetration depth were calculated at 4- and 6-mm levels from the root apex. Data from contact angle measurements, mechanical testing, and penetration assessment parameters were subjected to the independent samples t test with a significance level set at P < .05.ResultsA formulation of C-HA nanocomplexes (2 mg/mL) was chosen as a polyanionic, hydrophilic, nonaggregating concentration having bioactivity potential established through the formation of phosphate/carbonate bonds and the crystalline nature of the formed minerals. A significantly lower contact angle and higher UTS were registered for the C-HA nanocomplex–conditioned group (P < .05). Statistically significant (P < .05) greater sealer penetration was recorded at the 4-mm level for all assessment parameters and percent area penetration at 6 mm for the C-HA nanocomplex group.ConclusionsC-HA nanocomplex conditioning enhances dentin surface wettability to facilitate greater tricalcium silicate sealer penetration and UTS of dentin. |
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| Keywords: | Chitosan dentin nanocomplexes Sealer penetration depth ultimate tensile strength |
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