卵巢皮质玻璃化冷冻保存及移植的损伤因素

卵巢组织冻存和移植在技术上可行，有效性也随着技术发展而提高，可用于女性生育力保存，尤其是青春期前放化疗的患癌女性的生育能力保存。玻璃化冷冻被认为是目前最有潜力的卵巢组织冻存方法之一，但其安全性和有效性尚需进一步验证。冷冻损伤及移植缺血再灌注损伤是卵巢冻存和移植中需要克服的难题，如何尽可能有效地保存卵泡是难点及重点。从冷冻保护剂、卵巢组织大小、冷冻载体、抗氧化剂、促进新血管形成和激素6个方面对目前已有的玻璃化冷冻及移植条件、效果等进行了阐述，有益于女性生育力保存的项目推进。

Injury Factors of Vitrification Cryopreservation and Transplantation of Ovarian Cortex

The cryopreservation and transplantation of ovarian tissue is technically feasible, and the effectiveness is improved with the development of technology. It can be used to preserve female fertility, especially to preserve the fertility of prepubertal women with cancer who will have radiotherapy and chemotherapy. Vitrification is considered to be one of the most promising methods for cryopreservation of ovarian tissue, but its safety and efficacy need to be further verified. Cryogenic injury and transplantation ischemia-reperfusion injury are two problems of further development of cryopreservation and transplantation, in which the difficulty and focus are how to preserve follicles effectively. The existing freezing and transplantation conditions and effects are evaluated from six aspects of cryoprotectant, ovarian tissue size, cryocarrier, antioxidants, promoting angiogensis and hormones, in order to advance the project of female fertility preservation.