Release of Transforming Growth Factor Beta 1 from Human Tooth Dentin after Application of Either ProRoot MTA or Biodentine as a Coronal Barrier |
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| Affiliation: | 1. Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry, Seoul, Korea;2. Department of Conservative Dentistry, Wonju Severance Christian Hospital, Yonsei University, Wonju, Korea;3. Dongnam Regional Division, Korea Institute of Industrial Technology, Jinju, Korea;4. Department of Endodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;6. Department of Conservative Dentistry, School of Dentistry, Dental Research Institute, Pusan National University, Yangsan, Korea;5. Department of Electrical & Electronic Engineering, Yonsei University College of Engineering, Seoul, Korea;1. Department of Dental Biomaterials Science, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea;2. Department of Conservative Dentistry, Korea University Anam Hospital, Seoul, Korea;3. Department of Health Policy and Management, College of Health Science, Seoul, Korea;4. Department of Public Health Science, Graduate School, Korea University, Seoul, Korea;6. Special Care Clinic, Seoul National University Dental Hospital, Seoul, Korea;1. Department of Periodontics and Dental Hygiene, School of Dentistry, University of Texas Health Science Center at Houston, Houston, Texas;2. Department of Periodontics and Endodontics, Division of Endodontics, School of Dental Medicine, University at Buffalo, Buffalo, New York;3. Periodontics and Dental Implants, Houston, Texas;1. Department of Biology and Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia;2. Department of Pathophysiology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia;3. Department of Microbiology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia;4. Department of Restorative Odontology and Endodontics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia;6. Faculty of Medicine, Institute of Medical Statistics and Informatics, University of Belgrade, Belgrade, Serbia;5. Clinic of Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia;1. Department of Periodontics, Virginia Commonwealth University, Richmond, Virginia;2. Private Practice, Sacramento, California;3. Department of Oral and Maxillofacial Surgery, Virginia Commonwealth University, Richmond, Virginia;1. Department of Oncology, University of Turin, Turin, Italy;2. Department of Surgical Sciences, Dental School, University of Turin, Turin, Italy;3. Department of Medical Sciences, Division of Cardiology, Città della Salute e della Scienza di Torino, Turin, Italy;4. Department of Medical Sciences, Cancer Epidemiology Unit, University of Turin, Turin, Italy |
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| Abstract: | IntroductionVarious factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal barrier in the final step of regenerative endodontic procedures. The purposes of this study were to evaluate the effect of either MTA or BD after application as a coronal barrier on transforming growth factor beta 1 (TGF-β1) release from root canal dentin and to observe the impact of these materials on human apical papilla cell (APC) mineralization.MethodsEither MTA or BD was applied in enlarged root canals of human root segments. After storing for 14 days in phosphate-buffered saline, TGF-β1 release was evaluated using an enzyme-linked immunosorbent assay. To investigate the effect of the materials on APC mineralization, APCs were grown in the presence of either the materials alone or material-filled root segments. Cell mineralization was quantified after 14 and 21 days using alizarin red S staining. Calcium deposits were quantitatively analyzed. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests with the significance level at .05.ResultsThe greatest amount of TGF-β1 release was observed in the root segments treated with BD. BD, used either alone or as a coronal barrier, promoted greater APC mineralization than did MTA on both days 14 and 21. Interestingly, when BD was applied as a coronal barrier, the mineralization effect was significantly reduced compared with the use of the materials alone (P < .05).ConclusionsWhen used as a coronal barrier, BD promoted the release of TGF-β1 from the root canal dentin. A higher mineralization effect was observed with BD than with MTA. |
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| Keywords: | Biodentine coronal barrier differentiation intracanal calcification ProRoot MTA regenerative endodontic transforming growth factor beta 1 |
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