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不同牙龋病状况患者龈上菌斑及龋损组织微生物多样性分析
引用本文:王诗维,杨建军,张松梓. 不同牙龋病状况患者龈上菌斑及龋损组织微生物多样性分析[J]. 上海口腔医学, 2022, 31(2): 201-204. DOI: 10.19439/j.sjos.2022.02.017
作者姓名:王诗维  杨建军  张松梓
作者单位:西安医学院第一附属医院 口腔科,陕西 西安 710077
摘    要:目的: 探讨不同龋病状况患者龈上菌斑及龋损组织微生物的组成特点及多样性差异。方法: 随机双盲法选择2019年1月—12月于西安医学院第一附属医院口腔科确诊的龋病患者33例(轻度、中度、重度各11例),无龋病健康者10例。收集龈上牙菌斑和龋损组织,采用细菌DNA测序方法对细菌16S rRNA-cDNA高可变区进行测序,比较各组微生态种属和相对丰度。采用SPSS 23.0软件包对数据进行统计学分析。结果: 与无龋组相比,龋病组龈上菌斑、龋损组织中微生物含量和丰富度均显著降低(P<0.05)。轻、中、重度组患者龋损组织微生物主要为拟杆菌门、螺旋体门、变形菌门、梭杆菌门、厚壁菌门、放线菌门,各组菌属比例有显著差异(P<0.05)。轻、中、重度组患者龈上菌斑微生物主要有21种,其中,梭杆菌、科里亚细菌、奈瑟菌目、放线菌目、乳杆菌目占比高,其他均小于1%,各组5种菌属比例无显著差异(P>0.05)。结论: 龋病的发生由多菌种所致,且为多种细菌的共同作用。不同龋病状况患者龈上菌斑及龋损组织微生物丰度有显著差异,随着龋病的发生,微生物的多样性下降。

关 键 词:牙龋病  龈上菌斑  龋损组织  微生物  
收稿时间:2020-11-06
修稿时间:2021-04-06

Analysis of microbial diversity in supragingival plaque and caries tissue of patients with different dental caries phenotypes
WANG Shi-wei,YANG Jian-jun,ZHANG Song-zi. Analysis of microbial diversity in supragingival plaque and caries tissue of patients with different dental caries phenotypes[J]. Shanghai journal of stomatology, 2022, 31(2): 201-204. DOI: 10.19439/j.sjos.2022.02.017
Authors:WANG Shi-wei  YANG Jian-jun  ZHANG Song-zi
Affiliation:Department of Stomatology, First Affiliated Hospital of Xi'an Medical College. Xi'an 710077, Shaanxi Province, China
Abstract:PURPOSE: To identify the microbial characteristics and diversity in supragingival plaque and caries tissue of patients with different dental caries phenotypes. METHODS: From January 2019 to December 2019, randomized double-blind method was used to select 10 healthy people without caries and 33 patients with caries of mild, moderate and severe degrees in dental clinic of our hospital. Supragingival plaque and caries tissues were collected, and detected by pyrosequencing through amplification of the 16S rRNA-cDNA hypervariable regions. Then the microbial species and relative abundance were compared among patients with different severity degrees. SPSS 23.0 software package was used to analyze the data. RESULTS: Compared with non-caries group, the content and abundance of microorganisms in supragingival plaque and carious tissue of caries group were significantly decreased (P<0.05). The main caries tissue of three severity degree groups were dominated by Bacteroidetes, Spirochaetes, Proteobacteria, Fusobacteria, Firmicutes and Actinobacteria, and the proportion of the predominant bacteria had significant difference among three groups(P<0.05). There were 21 species of supragingival bacteria in three groups, among which Fusobacteriales, Coriobacteriales, Neisseriales, Actinomycetales and Lactobacillales accounted for a high proportion, and the remainings were all below 1%, while the proportion of five main bacteria showed no significant difference among three groups (P>0.05). CONCLUSIONS: Caries is caused by a variety of bacteria, and is the result of microbial communities rather than a single pathogen; Moreover, the microbial abundance of plaque and caries tissue vary among patients with different dental caries phenotypes, and the microbial diversity has a decreasing trend in the progress of dental caries.
Keywords:Dental caries  Supragingival plaque  Caries tissue  Microbe  
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