The Chlorella H+/hexose cotransporter gene

Summary The complete genomic sequence of the inducible Chlorella kessleri H⁺/hexose cotransporter (HUP1) has been obtained from two overlapping clones isolated from a gt10 library. The HUP1 gene is interrupted by 14 introns with the first intron being located in the 5-untranslated part of the gene. The average intron length is 220 bp, yielding a very regular intron/exon pattern in the gene. The codon usage in this gene is strongly biased with a clear preference for C and a strong suppression of A. A consensus sequence for a putative algal polyadenylation sequence is shown and compared with other algal cDNA sequences.