黄芩苷对庆大霉素致耳蜗螺旋神经节细胞损伤作用的影响

目的：探讨黄芩苷(baicalin，BA)对庆大霉素(gentamicin，GM)致小鼠耳蜗螺旋神经节(spiral ganglion，SG)细胞毒性的拮抗作用。方法：用MTT法检测庆大霉素对SG细胞的毒性作用以及黄芩苷对此作用的影响，用紫外分光光度法分别测定各实验组SG细胞总超氧化物歧化酶(T-SOD)活性，谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)含量。结果：黄芩苷可显著抑制GM所导致的SG细胞中T-SOD和GSH-PX活性降低及MDA含量升高。结论：黄芩苷通过清除耳蜗螺旋神经节细胞内氧自由基和抑制脂质过氧化反应，拮抗庆大霉素耳毒性作用。

Protective effect of baicalin-mediated on mice spinal ganglion cells against gentamicin-induced ototoxicity

AIM: To study the protective effect of baicalin (BA) against the ototoxicity of gentamicin (GM) of mice spinal ganglion (SG) cells. METHODS: MTT assay was employed to determine the growth inhibition of GM on the SG cells. The activities of superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), and the content of malondialdehyde (MDA) were measured by UV chromatography in SG cells from mice of different groups. RESULTS:Baicalin reversibly limited the GM-induced growth inhibition of SG cells in a dose-dependent manner. BA increased the activity of T-SOD and GM-induced decreased activity of T-SOD and GSH-PX in SG cells. The compound could also reduce MDA level and limite the GM-induced high content of MDA in SG cells. CONCLUSION: Baicalin mediated neuroprotective effect on SG cells is against gentamicin-induced ototoxicity through anti-oxidant.