HPLC测定钩藤药材中钩藤碱、异钩藤碱的含量 |
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引用本文: | 童宏龙,刘英波,李江,黄文荣,谭怀美. HPLC测定钩藤药材中钩藤碱、异钩藤碱的含量[J]. 中国民族民间医药杂志, 2012, 0(18): 56-57 |
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作者姓名: | 童宏龙 刘英波 李江 黄文荣 谭怀美 |
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作者单位: | [1]贵阳中医学院药学院,贵州贵阳550002 [2]遵义医药高等专科学校药学系,贵州遵义563000 [3]贵州百花医药股份有限公司,贵州遵义563000 |
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基金项目: | 国家“重大新药创制”科技重大专项一钩藤降压缓释制剂的研制. |
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摘 要: | 目的:用HPLC法建立钩藤药材中钩藤碱(Rhynchophylline)、异钩藤碱(Isorhynchophylline)的含量测定方法,用于钩藤药材的质量控制。方法:色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm),以甲醇:水=65:35(V/V,含0.01mmol/L三乙胺,冰醋酸调PH7.0)为流动相,流速为1.0 ml.min-1,在245nm检测波长下,测定钩藤药材中钩藤碱、异钩藤碱含量。结果:钩藤碱及异钩藤碱的回归方程分别为Y=1.0E+6x-21142和Y=1.1E+6x-13566,线性范围分别为0.254~1.524μg和0.216~1.296μg,相关系数分别0.9999和0.9999。结论:本法正确、简便、快速,对于控制和评价钩藤药材的品质有重要意义。
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关 键 词: | 钩藤 高效液相色谱法 钩藤碱 异钩藤碱 |
The study of the content determination of Rhynchophylline and Isorhynchophyiline in Uncaria Rhynchophylla by HPLC |
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Affiliation: | TONG Hong - long, LI Jiang, HUANG Wen - rong 1. GuiYang College of Traditional Chinese Medicine, Guiyang 550002, China; 2. Guizhou Baihua Pharmaceutical Co. , Ltd. , Zunyi 563000, China |
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Abstract: | Objective : To determine the contents of Rhynchophylline and Isorhynchophylline in Uncaria rhynchophylla ( Miq. ) Jacks. by the method of HPLC . Methods : Contents of the isoflavones ( Rhynchophylline and Isorhynchophylline) in Uncaria rhyn- chophylla ( Miq. ) Jacks. was measured by HPLC [ a column of Diamonsil C18 ( 4.6 mm x 250 mm, 5 um ), methanol: water = 65 : 35 ( V ! V, with 0. 01 mmol ! L triethylamine, glacial acetic acid adjusted PH7.0) as the mobile phase, flow rate 1.0 mL ~ min - 1, detective wave 245 nm] to determine the contents of Rhynchophylline and Isorhynchophylline. Results : The two regression e- quations are Y = 1.0E + 6x - 21142 and Y = 1.1E + 6x - 13566, and have good linearity in the range of 0. 254 - 1. 524 ug and 0. 216 - 1. 296 ug , r =0. 9999 and 0. 9999 respectively. Conclusion: The method is correct , simple, rapid, it is vital for control- ling and evaluating the quality of Chinese medicine Uncaria rhynchophylla ( Miq. ) Jacks. |
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Keywords: | Uncaria rhynchophylla (Miq.) Jacks. HPLC Rhynchophylline Isorhynchophylline |
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