| RNAi对人乳腺癌MCF-7细胞E2F1、TS基因表达的抑制作用 |
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| 引用本文： | 黄慧敏,李惠翔,张红,姜国忠,赵阿红.RNAi对人乳腺癌MCF-7细胞E2F1、TS基因表达的抑制作用[J].山东医药,2008,48(43):8-9. |
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| 作者姓名： | 黄慧敏 李惠翔 张红 姜国忠 赵阿红 |
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| 作者单位： | 1. 河源市人民医院,广东河源,517000
2. 郑州大学第一附属医院 |
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| 摘 要： | 目的观察RNA干扰（RNAi）技术对乳腺癌细胞MCF-7中E2F1、胸苷酸合成酶（TS）基因表达的靶向抑制作用。方法设计合成并构建重组质粒pSIREN-E2F1，将重组质粒转染MCF-7细胞。筛选稳定转染的单克隆细胞扩大培养，应用RT-PCR方法检测各组细胞E2F1、TS mRNA表达情况。结果转染组E2F1、TS基因表达均受到抑制：结论RNAi技术特异、高效抑制靶基因E2F1及TS表达。
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| 关 键 词： | E2F1基因 胸苷酸合成酶基因 RNA干扰 肿瘤抑制 |
Inhibition effect of RNAi to E2F1 and TS genes expression in human breast cancer MCF-7 cell |
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| HUANG Hui-min,LI Hui-xiang,ZHANG Hong,JIANG Guo-zhong,ZHAO A-hong.Inhibition effect of RNAi to E2F1 and TS genes expression in human breast cancer MCF-7 cell[J].Shandong Medical Journal,2008,48(43):8-9. |
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| Authors: | HUANG Hui-min LI Hui-xiang ZHANG Hong JIANG Guo-zhong ZHAO A-hong |
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| Abstract: | Objective To deect the inhibition of RNAi techinque to the expression of E2F1gene in breast cancer cell line MCF-7 and investigate the expression of TS gene. Method Recombinant plasmid pSIREN-E2F1 was constructed and transfected into human breast cancer cell line MCF-7. And then selected the stable transfected cells scaling up culture. The expression of E2F1 and TS gene were detected by RT-PCR. Result The expression of E2F1mRNA and TSmRNA were suppressed in pSIREN-E2F1 transfected cells.Conclusion RNAi can ... |
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| Keywords: | E2F1 gene thymidylate synthase gene RNA inhibition tumor suppressor |
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