动脉瘤性蛛网膜下腔出血患者脑脊液IL-1β、IL-6及TNFα检测及意义

【目的】探讨动脉瘤性蛛网膜下腔出血（SAH）患者脑脊液白介素-1β（IL-1β）、白介素-6（IL-6）和肿瘤坏死因子d（TNFa）水平及其与脑血管痉挛的关系。【方法】选用双抗体夹心酶联免疫吸附试验（ELISA）检测动脉瘤性SAH患者脑脊液IL-1β、IL-6和TNFa浓度，并将其结果与经颅多普勒（TCD）检查判别的脑血管痉挛与否进行相关分析。【结果】动脉瘤性SAH患者脑脊液中IL-1β、IL-6和TNFa水平均明显升高（P〈0．05），IL-1β、IL-6水平与脑血管痉挛显著相关（P〈0．05），TNFa水平与脑血管痉挛无统计学相关（P〉O．05）。【结论】动脉瘤性SAH患者脑脊液IL-1β和IL-6水平增高可能与脑血管痉挛发病有关，检测其患者脑脊液中IL-1β和IL-6有助于脑血管痉挛的预测及治疗。

Measurement of IL-1β, IL-6 and TNF-a Levels in Cerebrospinal Fluid of Patients with Aneurysmal Subarachnoid Hemorrhage and Its Significance

Objective]To explore the relationship of the levels of interleukin lbeta（IL-1β）, interleukin-6（IL-6） and tumor necrosis factor-alpha（TNF-a） in cerebrospinal fluid of patients with aneurysmal subarachnoid hemorrhage（SAH） with cerebral vasospasrn. Methods] The levels of IL-1β, IL-6 and TNF-a in cerebrospinal fluid of patients with aneurysmal SAH were measured by double-antibody sandwich ELISA. The relationship between detection results and cerebral vasospasm diagnosed by transcranial doppler（TCD） was analyzed. Results]The levels of IL-1β, IL-6 and TNF-a in patients with aneurismal SAH significantly increased（ P 〈0.05）. The levels of IL-1β and IL-6 were significantly related to cerebral vasospasm（ P〈0.05）, while the level of TNF-a was not significantly related to cerebral vasospasm（ P〉0.05 ）. Conclusion]The increased levels of IL-1β and IL-6 in cerebrospinal fluid of patients with aneurysmal SAH may be related to the pathogenesis of cerebral vasospasm. The measurement of IL-1β and IL-6 in cerebrospinal fluid of patients with aneurysmal SAH can be useful in prediction and treatment of cerebral vasospasrn.