重组人甲状旁腺激素(1-34)对体外培养成骨细胞骨形成的作用

目的 体外观察重组人甲状旁腺激素[recombinant human parathyroid hormone（1-34），rhPTH（1-34）]对成骨细胞骨形成的刺激作用。方法采用酶消化法分离获得大鼠成骨细胞，间隙加药方法给予rhPTH（1-34），观察药物对成骨细胞增殖、分化及矿化功能的影响。结果 10^-12～10^-8mol／L浓度对成骨细胞的增殖、分化和矿化功能均显示刺激作用。10^-11～10^-8mol／L浓度组的MTT、ALP的A值及矿化结节形态计量结果与对照组比较，具有显著性增加（P〈0．05），增加率以10^-9mol／L浓度组为最高，并呈剂量一效应关系。结论 rhPTH（1-34）对体外培养成骨细胞的增殖、分化和矿化功能具有明显促进作用。

Effects of bone formation on culture osteoblasts with rhPTH(1-34) in vitro

Purpose To investigate the stimulating effects of recombinant human parathyroid hormone(1-34)rhPTH(1-34) on bone formation of the osteoblasts in vitro. Methods The osteoblasts were isolated from fetal rats by enzymatic-digestion,then given rhPTH(1-34) in intermission. The effect of rhPTH(1-34) on proliferation,differentiation and mineralization of osteoblasts was observed. Results rhPTH(1-34) in the concentration from 10~ -12 mol/L to 10~ -8 mol/L could enhance the proliferation differentiation and mineralization of osteoblasts. MTT,ALP,the value of A and the numbers of mineralized nods from 10~ -11 mol/L to 10~ -8 mol/L were apparently increased compared to the control (P<0.05),especially at 10~ -9 mol/L. Conclusions rhPTH(1-34) has obvious promoting effect on proliferation differentiation and mineralization of osteoblasts in vitro.