生活饮用水中五种人工合成甜味剂的固相萃取-高效液相色谱-质谱同时测定法

目的建立生活饮用水中安赛蜜、三氯蔗糖、阿斯巴甜、糖精钠、甜蜜素5种人工合成甜味剂的固相萃取-液相色谱-质谱(HPLC-MS-MS)测定法。方法采用HLB固相萃取(SPE)柱对生活饮用水中人工合成甜味剂进行提取和富集后,以ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.7μm)为分析柱,以乙腈-10 mmol/L乙酸铵(含2 mmol/L Tris)为流动相进行梯度洗脱,以负离子喷雾模式电离,多反应离子监测方式进行定性及定量检测。结果在5~200μg/L的线性范围内,5种人工合成甜味剂的回归方程均呈较好的线性关系,r0.999 6。该方法的检出限为0.2~8.0 ng/L,平均回收率为82.1%~96.3%,RSD为3.9%~9.0%。结论该方法操作简单、快捷,灵敏度和准确度均较高,精密度较好,适用于生活饮用水中5中人工合成甜味剂的测定。

Simultaneous determination of five artificial sweeteners in drinking water by solid-phase extraction and liquid chromatography-tandem mass spectrometry

Objective To establish a method based on solid-phase extraction and liquid chromatography-mass spectrometry for simultaneous determination of five artificial sweeteners in drinking water.Methods The water samples were passed through HLB cartridges to extract and concentrate the sweeteners,and the target compounds were separated by an ACQUITY UPLC HSS T3 column (100 mm×2.1 mm,1.7 μm),acetonitrile and 10 mmol/L ammonium acetate (containing 2 mmol/L Tris) solution were used as the mobile phase by gradient elution,mass spectrometry under negative electrospray ionization mode and multiple reaction monitoring (MRM) were performed for the qualitative and quantitative analysis.Results In the linear range of 5-200 μg/L,the regression equation of the five artificial sweeteners showed a good linear relationship (r ＞0.999 6).The limit of detection was in the range of 0.2-8.0 ng/L,the average recovery rates were in the range of 82.1％-96.3％,and the RSDs were between 3.9％ and 9.0％.Conclusion The method is simple,fast,sensitive,accurate and applicable to simultaneous determination of five artificial sweeteners in drinking water.