地黄饮子对脑缺血再灌注大鼠海马脑源性神经营养因子和基质细胞衍生因子表达的影响

目的 探讨地黄饮子对脑缺血冉灌注大鼠海马脑源性神经营养因子(brain derived neurotrophic factor,BDNF)和基质细胞衍生因子(stromal cell derived factor 1,SDF1)的表达及修复神经元的作用机制.方法 选取清洁级SD大鼠75只,按数字表法随机分为正常组、治疗组和缺血组,每组25只.10％水合氯醛麻醉,暴露并钳夹双侧颈总动脉40 min,造模使大鼠急性脑缺血.治疗组:造模后灌服地黄饮子汤36 g/kg;缺血组:造模后每日胃灌盐水1次;正常组:正常饮食.3周后,测量大鼠脑梗死面积,分别测定3组大鼠脑海马CA1区BDNF和SDF1蛋白表达情况.结果 正常组海马CA1区BDNF(99.25±9.48)和SDF1蛋白含量[(2.59±0.76) mg/L]均明显高于地黄饮子治疗组[BDNF:(79.98±10.68),SDF1蛋白含量:(3.68±0.96) mg/L]]和缺血组[BDNF:(59.59±13.73),SDFl蛋白含量:(0.93±1.24)mg/L],差异有统计学意义(P＜0.05).地黄饮子治疗组BDNF和SDF1蛋白含量明显高于缺血组,差异有统计学意义(P＜0.05).治疗组的脑梗死面积明显低于缺血组,差异有统计学意义(P＜0.05).结论 地黄饮子治疗可以提高BDNF和SDF1蛋白水平,增加内源性保护机制和神经干细胞增殖、迁移,保护神经元的功能.

Expression and significance of brain derived neurotrophic factor and stromal cell derived factor 1 in the hippocampus of rats with cerebral ischemia reperfusion

Objective To explore the expression of brain derived neurotrophic factor (BDNF) and stromal cell derived factor 1 (SDF1) in the hippocampus of rats following cerebral ischemia reperfusion,and the mechanism involved in the repair of neurons.Methods Seventy-five SD rats were randomly divided into the normal group,the treatment group and the ischemia group,each consisting of 25 animals.Chloral hydrate at a concentration of 10％ was used to induce anesthesia.Bilateral common carotid artery was exposed and clipped for 40 minutes to develop an acute cerebral ischemia model.Following development of the model,the treatment group was given rehmannia Yinzi liquid by gavage at a dosage of 36 g/kg,while the ischemia group received physiological saline also by gavage once a day.The normal group was given normal feed.Three weeks later,the areas of cerebral infarction were detected,and the expression levels of BDNF and SDF1 were respectively detected in CA1 of the hippocampus.Results The levels of BDNF (99.25 ± 9.48) and SDF1 [(3.68 ± 0.96) mg/L] in CA1 in the animals of the normal group were all significantly higher than those of the treatment group [(79.98 ± 10.68) and (2.59 ± 0.76) mg/L] and the ischemia group [(59.59 ± 13.73) and (0.93 ± 1.24) mg/L],and statistical significance could be seen,when comparisons were made between them (P ＜0.05).The levels of BDNF and SDF1 in the rehmanniae Yinzi treatment group were also obviously higher than those of the ischemic group,also with statistical significance (P ＜O.05).The area of cerebral infarction of the treatment group was significantly smaller than that of the ischemia group,also with statistical significance (P ＜ 0.05).Conclusion Rehmannia Yinzi liquid could increase the levels of BDNF and SDF1,improve the endogenous protective effect,enhance the proliferation and migration of stem cells,and had the function to protect neurons.