邻苯二甲酸二(2-乙基)己酯青春前期暴露对雌性大鼠性激素水平及卵巢抗氧化系统的影响

目的研究邻苯二甲酸二(2-乙基)己酯(di-(2-ethylhexyl)phthalate,DEHP)青春前期暴露对雌性大鼠性激素水平及卵巢氧化应激指标的影响。方法将40只健康3周龄SPF级雌性SD大鼠按体重随机分为5组,分别为对照(玉米油)组和0.5、5、50、500 mg/kg DEHP暴露组,每组8只。采用经口灌胃方式进行染毒,染毒容量为1 ml/kg,每天1次,连续染毒4周。测定雌性大鼠血清、睾丸氧化应激[丙二醛(MDA)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)]指标以及血清、海马性激素[雌二醇、卵泡刺激素(FSH)、黄体生成素(LH)]水平。结果与对照组相比,500 mg/kg DEHP暴露组雌性大鼠卵巢MDA、ACP水平增加,差异均有统计学意义(P0.05);而各剂量DEHP暴露组雌性大鼠卵巢SOD、CAT和AKP的水平均未见明显变化。各剂量DEHP暴露组雌性大鼠血清MDA、SOD、CAT、ACP和AKP的水平与对照组相比,差异均无统计学意义(P0.05)。与对照组相比,仅0.5 mg/kg DEHP暴露组雌性大鼠海马雌二醇水平及5 mg/kg DEHP暴露组雌性大鼠血清雌二醇水平降低,差异均有统计学意义(P0.05)。与对照组相比,仅500 mg/kg DEHP暴露组雌性大鼠血清LH水平升高,差异有统计学意义(P0.05);而各剂量DEHP暴露组雌性大鼠血清FSH水平均未见明显变化。结论 DEHP青春前期暴露会导致雌性大鼠卵巢氧化应激损伤,并易引起青春期性激素分泌紊乱。

Effects of prepubertal exposure to DEHP on sex hormone levels and ovarian oxidative stress levels in infant female rats

Objective To investigate the effects of prepubertal exposure to di-(2-ethylhexyl) phthalate (DEHP) on sex hormone levels and ovarian oxidative stress levels in infant female rats.Methods Forty SPF female SD rats aged three weeks were randomly divided into solvent (peanut oil) control group and four DEHP groups (0.5,5,50 and 500 mg/kg body weight),eight in each group,treated through gavage,once a day for four consecutive weeks.The hippocampal and serum oxidative stress levels [malondialchehyche(MDA),super oxide dismutase(SOD),acid phosphatase(ACP),alkaline phosphatase(AKP) and catalase(CAT)] were measured,the hippocampal and serum sex hormones [estrogen(E2),follicule-stimulating hormone (FSH),luteinizing hormone (LH)] were also determined.Results Compared with the solvent control group,the contents of MDA and the activities of ACP in 500 mg/kg body weight group increased significantly (P＜0.05);No changes were observed in the levels of MAD,SOD,CAT,ACP and AKP in all DEHP-treated groups.The hippocampal E2 levels of 0.5 mg/kg body weight group and the serum E2 levels in 5 mg/kg body weight group were significantly lower than those in the solvent control group (P＜0.05).The contents of serum LH of 500 mg/kg body weight group were significantly higher than that in the solvent control group (P＜0.05).However,no significant changes in the levels of serum FSH were observed in all DEHP-treated groups.Conclusion Prepubertal exposure to DEHP may induce ovarian oxidative damage and lead to sex hormone secretion disorder.