纳米二氧化硅和纳米氧化锌颗粒对人肝癌细胞的毒性作用

目的探讨纳米二氧化硅和纳米ZnO颗粒对人肝癌(HepG2)细胞毒性的影响。方法将处于对数生长期的细胞分别暴露于含终浓度分别为0(对照)~200μg/ml纳米二氧化硅、纳米ZnO颗粒的无血清培养基中培养24 h。检测细胞活性及细胞膜的完整性。结果与对照组比较,各浓度纳米二氧化硅及25~200μg/ml纳米ZnO染毒组HepG2细胞的存活率均较低,差异有统计学意义(P0.05);且随着染毒浓度的升高,纳米二氧化硅和纳米ZnO染毒HepG2细胞的存活率均呈下降趋势。与相同浓度纳米二氧化硅相比,50~200μg/ml纳米ZnO染毒组HepG2细胞的存活率均较高,差异有统计学意义(P0.05)。与对照组比较,各浓度纳米二氧化硅及纳米ZnO染毒组HepG2细胞上清液中LDH的活力均较高,差异有统计学意义(P0.05);且随着纳米二氧化硅和纳米ZnO染毒浓度的升高,HepG2细胞上清液中LDH的活力均呈上升趋势。与相同浓度纳米二氧化硅相比,各浓度纳米ZnO染毒组HepG2细胞上清液中LDH的活力均较低,差异有统计学意义(P0.05)。结论两种纳米颗粒均可对HepG2细胞产生毒性作用,且纳米二氧化硅比纳米ZnO颗粒的细胞毒性作用更强。

Toxic effect of nano-silicon dioxide and nano-zinc oxide particles on HepG2 cells

Objective To understand the toxic effect of nano-SiO2 and nano-ZnO particles on HepG2 cells.Methods The cells in logarithmic growth phase were respectively exposed to serum-free medium containing 0 (control) to 200 μg/ml silica nanoparticles and ZnO nanoparticles for 24 hours.The cell activity and integrity of the cell membrane were detected.Results The survival rates of HepG2 cells in all nano-silica groups and 25 to 200 μg/ml nano-ZnO groups were significantly lower compared with the control group (P＜0.05);With the increase of exposure concentrations,the survival rates of HepG2 cells innano-silica and nano-ZnO group showed downward trend.The survival rates of HepG2 cells in 50 to 200 μg/ml nano-ZnO group were significantly higher than those in the same concentration of nano-silica group (P＜0.05).The activities of LDH in the supernatant of HepG2 cells in each group of nano-silica and nano-ZnO were statistically higher compared with the control group(P＜0.05);With the increase of exposure concentrations,the activities of LDH in the supernatant of HepG2 cells showed up trend.The activities of LDH in the supernatant of HepG2 cells in all nano-ZnO groups were significantly lower than that the same concentration of nano-silica group (P＜0.05).Conclusion SiO2 and ZnO nanoparticles may produce toxic effects on HepG2 cells,the nano-SiO2 showed stronger toxicity to HepG2 cells compared with nano-ZnO particles.