CRISPLD2蛋白对内毒素急性呼吸窘迫综合征小鼠的保护作用

目的 探讨CRISPLD2蛋白对内毒素急性呼吸窘迫综合征(acute respiratory disease syndrome,ARDS)小鼠的保护作用.方法 采用数字表法,将120只健康雄性昆明小鼠随机分为对照组(A组,40只):气道内滴入LPS(5 mg/kg);LPS预处理组(B组,40只):先予小鼠LPS(2 mg/kg)腹腔内注射,注射后第8天气道内滴入LPS(5 mg/kg);重组CRISPLD2处理组(C组,40只):先予小鼠尾静脉注射重组CRISPLD2(50 mg/kg),6 h后气道内滴入LPS(5 mg/kg).(1)采用数字表法将30只小鼠随机分成A1组、B1组、C1组(各组10只),气道滴入LPS 6 h后处死,测定肺湿干重比(W/D)、通透指数(LPI).(2)采用数字表法将90只小鼠随机分成A2组、B2组、C2组(各组20只),观察5 d内小鼠死亡情况,描绘生存曲线.结果 B、C两组W/D、LPI及5 d死亡率显著低于A组,差异有统计学意义(P<0.05);B组各项检测结果低于C组,差异有统计学意义(P>0.05).结论 提升CRISPLD2蛋白血浓度对内毒素所致ARDS具有保护作用,可降低肺损伤程度,改善肺毛细血管通透性,降低死亡率.

Protective effect of CRISPLD2 protein on acute respiratory disease syndrome induced by lipopolysaecharide in mice

Objective To investigate the protective effect of CRISPLD 2 protein on acute respiratory disease syndrome ( ARDS) induced by lipopolysaecharide ( LPS) in mice.Methods Healthy male Kunming mice were randomly divided into 3 groups:the control group (or group A) which received lipopolysaecharide via the intratracheal instillation at a dosage of 5 mg/kg, the LPS pre-conditioning group (or group B) which had intraperitoneal injection of LPS at a dosage of 2mg/kg and at day 8 received LPS via intra-tracheal instillation also at a dosage of 5mg/kg.The recombinant CRISPLD2 preconditioning group ( or group C) which was injected with recombinant CRISPLD2 at a dosage of 50mg/kg via cauda vein, and then at hour 6 received LPS via intratracheal instillation at the same dosage.(1)Thirty mice were randomly divided into group A1, group B1 and group C1, each consisting of 10 animals.The mice were sacrificed 6 hours after LPS intratracheal instillation .Then, W/D and LPI were measured.(2) Ninety mice were randomly divid-ed into group A2, group B2 and group C2, each consisting of 30 animals.The death of the animals in each group was closely observed and the survival curve was described .Results W/D, lung index and mortality after 5 days in group B and C were significantly lower , as compared with those of group A (P<0.05).The detected data of various indexes in group B were lower than those in group C (P>0.05).Conclusion Increased serum concentration of CRISPLD2 could obviously produce protective effect on ARDS induced by LPS , lighten injury on the lungs and improve permeability of pulmonary capillary and decrease mortality .