胸腺瘤细胞中PCNA、HSP70的表达水平的研究及意义

目的研究PCNA、HSP70在胸腺瘤细胞及正常胸腺组织中的表达水平及其临床意义。方法用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶染色法(S-P法)检测20例恶性胸腺瘤、18例良性胸腺瘤及12例正常胸腺组织细胞中PCNA、HSP70蛋白的表达水平。结果20例恶性胸腺瘤中PCNA、HSP70蛋白阳性表达分别为80.0%(1620)、75.0%(1520);18例良性胸腺瘤中PCNA、HSP70蛋白阳性表达分别为38.9%(718)、27.8%(518);12例正常胸腺组织细胞中分别为8.3%(112)、0(012),PCNA蛋白及HSP70蛋白的表达在良、恶性胸腺瘤和正常胸腺组织三者之间差异都有显著性(P<0.001),且在良、恶性胸腺瘤之间相比差异也均有显著性(P<0.05)。但良性胸腺瘤和正常胸腺组织相比,PCNA及HSP70蛋白的表达差异均无显著性(P>0.05)。结论通过检测HSP70与PCNA在胸腺瘤的表达水平,可以判断胸腺瘤的良、恶性,为术后进一步治疗提供了有价值的参考指标。

Expression and significance of PCNA and HSP70 in thymoma

Objective To study the expression and significance of PCNA and HSP70 in thymoma and normal thymus. Methods Immunohistochemical method (S-P) was used to examin the smears from 20 malignant thymoma,18 benign thymomas and 12 normal thymus. Results The positive rate of PCNA expression were 80.0%(16/20),38.9%(7/18) and 8.3%(1/12), and the positive rate of HSP70 expression were 75.0%(15/20),27.8%(5/18),0(0/12) in the three groups respectively. There were significant difference among the three groups in PCNA and HSP70 expression (P<0.001). Conclusions PCNA and HSP70 are the helpful marker for the diagnosis of malignant thymoma and benign thymoma.