脑深部电刺激伏核对吗啡成瘾依赖大鼠学习记忆的影响

目的 探讨脑深部电刺激(DBS)伏核对吗啡成瘾依赖大鼠学习记忆的影响.方法 通过DBS电极刺激吗啡成瘾依赖大鼠双侧伏核,于吗啡注射第10天和刺激结束后第1天,进行Morris水迷宫实验比较假刺激组、DBS组、生理盐水组(NS组)大鼠学习记忆能力的变化.结果 刺激前3组间的平均潜伏期总体比较差异有显著性(F=7.49,P=0.0077),吗啡依赖组大鼠的平均潜伏期明显长于NS组(P<0.01);刺激后3组间的平均潜伏期总体比较差异有显著性(P=7.76,P=0.0069),刺激后DBS组大鼠的平均潜伏期缩短,与假刺激组相比差异具有显著性(P=0.014),较NS组差异无显著性(P=0.43);假刺激组与NS组相比差异具有显著性(P=0.0056).刺激前NS组(51.94±3.06)与吗啡依赖组穿越Ⅱ象限时间差异有显著性(P=0.001);刺激后NS组(48.53±2.29)s]较假刺激组(20.74±2.13)s]差异有显著性(P=0.001),较DBS组(38.34±1.68)s]差异无显著性(P=0.062).结论 脑深部电刺激伏核改善了已被吗啡损害的大鼠的学习记忆功能.

Effect of deep brain stimulation in nucleus accumbens on learning and memory ability of morphine dependence rats

Objective To explore the effect of deep brain stimulation(DBS)in nucleus accumbens on learning and memory ability of morphine dependence rats.Methods According to stereotaxic atlas of the rat brain,DBS electrodes were implanted in rats bilateral nucleus accumbens,then Morris water maze test were carried out on the tenth day of morphine injection and the first day after DBS.Results The average time of reaching platform of three groups was different significantly before DBS(F=7.49,P=0.0077);and that of morphine addiction group wag longer than saline control group(P＜0.01).While after DBS,there was also significantly different(F=7.76,P=0.0069);the time had significant difference between DBS group and fake stimulation group(P=0.014),not saline control group(P=0.43);and fake stimulation group compared with saline control group(P=0.0056).The time of crossing the platform quadrant(Ⅱ)showed significant difference between saline control group and morphine addiction group(P<0.01);and the saline control group(48.53±2.29),compared with fake stimulotion group(20.74±2.13)(P=0.001),DBS group(38.34±1.68)(P=0.062).Conclusion DBS can improve the learning and memory ability impaired by morphine in rats.