| 淋病奈瑟菌外膜蛋白与粘膜佐剂的融合表达 |
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| 引用本文： | 潘静,毛旭虎,张卫军,鲁东水,罗萍,王宁.淋病奈瑟菌外膜蛋白与粘膜佐剂的融合表达[J].中华男科学杂志,2004,10(4):269-271,274. |
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| 作者姓名： | 潘静 毛旭虎 张卫军 鲁东水 罗萍 王宁 |
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| 作者单位： | 第三军医大学医学检验系,重庆,400038 |
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| 基金项目： | 重庆市应用基础研究基金 |
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| 摘 要： | 目的 :在大肠埃希菌 (E .coli)中表达淋病奈瑟菌外膜蛋白与粘膜免疫佐剂的融合蛋白。 方法 :PCR扩增淋病奈瑟菌保守抗原表位porinloopⅥ～Ⅷ (PL678)基因 ,并与不耐热肠毒素B亚单位 (LTB)融合 ,转化E .coli,ELISA、SDS PAGE及Western印迹分析其表达。 结果 :融合蛋白获得表达 ,具有结合GM1神经节苷酯的能力和与抗淋病奈瑟菌多克隆抗体的反应性。 结论 :融合蛋白的表达为研究淋病奈瑟菌分子内佐剂疫苗奠定了基础。
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| 关 键 词： | 淋病奈瑟菌 不耐热肠毒素B亚单位 融合表达 |
| 文章编号： | 1009-3591(2004)04-0269-04 |
| 修稿时间： | 2003年2月19日 |
Fusion Expression of Neisseria gonorrhoeae Outmembrane Protein with a Mucosal Adjuvant |
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| Pan Jing,Mao Xuhu,Zhang Weijun,Lu Dongshui,Luo Ping,Wang NingCollege of Medical Laboratory,Third Military Medical University,Chongqing ,China.Fusion Expression of Neisseria gonorrhoeae Outmembrane Protein with a Mucosal Adjuvant[J].National Journal of Andrology,2004,10(4):269-271,274. |
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| Authors: | Pan Jing Mao Xuhu Zhang Weijun Lu Dongshui Luo Ping Wang NingCollege of Medical Laboratory Third Military Medical University Chongqing China |
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| Institution: | College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China. |
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| Abstract: | OBJECTIVE: To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant. METHODS: The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot. RESULT: Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae. CONCLUSION: The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae. |
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| Keywords: | Neisseria gonorrhoeae subunit B of heat labile enterotoxin fusion expression |
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