淫羊藿苷上调NRF2改善SHR大鼠勃起功能

目的:研究淫羊藿苷是否通过调节核因子类红细胞2-相关因子2(NRF2)通路改善自发性高血压大鼠(SHR)的勃起功能。方法:随机将10周龄健康雄性WKY大鼠(WKR)与雄性SHR大鼠分为4组(每组6只,共24只):WKY对照组、WKR+淫羊藿苷组10 mg/(kg·d)灌胃]、SHR对照组,SHR+淫羊藿苷组10 mg/(kg·d)灌胃]。4周后,测定大鼠阴茎海绵体最大海绵体内压/平均动脉压(ICPmax/MAP)和阴茎海绵体中NRF2、HO-1、eNOS、P-eNOS、PPAR-γ和DDAH等的含量。结果:各组大鼠体重、血清睾酮水平无明显差异。SHR+淫羊藿苷组中阴茎海绵体组织的ICPmax/MAP、NRF2、HO-1、P-eNOS/eNOS、PPAR-γ、DDAH、NO和cGMP表达显著高于SHR对照组(P<0.05),但显著低于WKR对照组。SHR+淫羊藿苷治疗组阴茎海绵体组织的ADMA表达较SHR对照组显著降低(P<0.05)。结论:淫羊藿苷通过上调大鼠阴茎海绵体NRF2表达,上调HO-1、DDAH和PPAR-γ,抑制ADMA和增加P-eNOS/eNOS比值而改善SHR的勃起功能。

Icariin improves erectile function in spontaneously hypertensive rats by up-regulating Nrf2

Objective:To investigate whether icariin improves erectile function in spontaneously hypertensive rats(SHR)by regulating the NRF2 pathway.Methods:We equally randomized 24 ten-week-old healthy male Wistar-Kyoto rats(WKR)and healthy male SHR rats into four groups:WKR control,WKR+icariin,SHR control and SHR+icariin,the controls treated intragastrically with normal saline,and the animals in the WKR+icariin and SHR+icariin groups with icariin,all at 10 mg/kg/d for 4 weeks.Then we obtained the body weight and serum T level of the rats and measured the maximum intracorporeal pressure/mean arterial pressure(ICPmax/MAP)and the contents of nuclear factor-erythroid 2-related factor-2(Nrf2),memeoxygenase-1(HO-1),endothellial nitric oxide synthase(eNOS),peroxisome proliferator activated receptor gamma(PPAR-γ),dimethylarginine dimethylaminohydrolase(DDAH),nitric oxide(NO),cyclic guanosine monophosphate(cGMP)and asymmetric dimethylarginine(ADMA)in the corpora cavernosa tissue.Results:There were no statistically significant differences in the body weight or serum T level among the four groups of rats.The ratios of ICPmax/MAP and P-eNOS/eNOS and the expressions of Nrf2,HO-1,PPAR-γ,DDAH,NO and cGMP in the corpora cavernosa tissue were significantly higher in the SHR+icariin group than in the SHR control(P<0.05)but lower than in the WKR control.The ADMA level in the corpora cavernosa tissue was remarkably reduced in the SHR+icariin group compared with that in the SHR control(P<0.05).Conclusion:By up-regulating the expression of Nrf2,icariin increases the HO-1,DDAH and PPAR-γlevels and the P-eNOS/eNOS ratio in the corpora cavernosa and improves the erectile function of spontaneously hypertensive rats.