黄荆子乙酸乙酯提取物对人乳癌细胞体内外实验的研究

目的研究黄荆子乙酸乙酯提取物（EVn-50）在体内外对人乳癌MDA—MB-435S细胞的抑制作用。方法软琼脂克隆形成法测定细胞生长：溴化尿嘧啶（BrdU）掺入法检测细胞核酸的合成；建立MDA—MB-435S裸鼠异种移植瘤模型，观察经EVn-50处理后的肿瘤抑制率。结果在体外，随EVn-50浓度增高，细胞集落形成率明显下降（P〈0．01）；EVn-50对MDA—MB-435S细胞核酸合成具有抑制作用，呈剂量依赖性，其中高剂量组的BrdU掺入抑制作用最强，IC50为16．8ug／mL。在体内，EVn-50抑制MDA—MB-435S细胞裸鼠异种移植瘤生长，20、40、80ug／mL的EVn-50对移植瘤的瘤重抑制率分别为18％、31％和63％，呈浓度依赖性。结论EVn-50在体内外对MDA—MB-435S细胞均具有生长抑制作用。

Experimentation of the extract from semen viticis negundo with acetoactate on human breast cancer MDA-MB-435S cell in vitro and in vivo

Objective To study the inhibition of EVn-50 on human breast cancer cells MDA-MB-435S in vitro and in vivo. Methods Soft agar clone formation assay was carried out to detect the growth inhibitory effect of cells. Nucleic acid synthesis of MDA-MB-435S cells was determined by B rdU incorporate assay.The established of human breast cancer xenografis in nude mouse model was used to test inhibition rate of EVn-50 on human breast cancer. Results In vitro, With concentration higher, The colonyforming rate was reduced drastically （P〈0.01）;EVn-50 significantly inhibited nucleic acid synthesis of MDA-MB-435S cells,in a concentration dependentmanner; and the highest concentration was the most effective, its IC50 was 16.8tzg/mL. In vivo, EVn-50 inhibited the growth of human breast carcinoma xenografts in nude mice. The tumor weight inhibitory rate of EVn-50 at 20,40,80ug/mL was l 8%,31% ,63% respectively, in a dose-dependent manner. The pathological alterations were observed. In EVn-50 groups, degrees of necrosis in tumor tissues were markedly increased respectively compared with the model group. Conclusion EVn-50 can significantly inhibit the growth and proliferation of breast cancer cells MDA-MB-435S in vitro and in vivo.