| 青年及老年大鼠下丘脑弓状核阿黑皮素原mRNA的表达 |
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| 引用本文： | 向正华,孟璘,蒋玲,钱国桢. 青年及老年大鼠下丘脑弓状核阿黑皮素原mRNA的表达[J]. 第二军医大学学报, 2001, 22(5): 481-483 |
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| 作者姓名： | 向正华 孟璘 蒋玲 钱国桢 |
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| 作者单位： | 1. 第二军医大学基础医学部组织胚胎学教研室,
2. 上海理工大学电气工程学院, |
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| 基金项目： | 国家自然科学基金资助项目 ( 5 2 73 880 5 2 8) |
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| 摘 要： | 目的：观察青年和老年期大鼠下丘脑弓状核阿黑皮素原mRNA（POMC mRNA）的表达水平。方法应用原位杂交技术显示POMCmRNA和用计算机图像分析观察杂交信号的强弱及阳性细胞的面积，结果：与青年组相比，老年组下丘脑弓状核及其附近区域POMCmRNA阳性神经元的灰度下降，POMCmRNA阳性神经元的数目无明显变化，结论：老年期POMCmRNA阳性神经元相应的mRNA表达水平下降可能是下丘脑的POMC基因衍生肽表达水平下降的原因之一。
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| 关 键 词： | 前阿片黑素细胞皮质激素 原位杂交 计算机辅助图像处理 下丘脑 弓状核 大鼠 mRNA |
| 文章编号： | 0258-879X(2001)05-0481-03 |
| 修稿时间： | 2000-09-20 |
Expression of pro-opiomelanocortin mRNA in the hypothalamic arcuate nucleus in young and senile rats |
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| XIANG Zheng-Hua,MENG Lin,JIANG Ling,QIAN Guo-Zhen. Expression of pro-opiomelanocortin mRNA in the hypothalamic arcuate nucleus in young and senile rats[J]. Former Academic Journal of Second Military Medical University, 2001, 22(5): 481-483 |
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| Authors: | XIANG Zheng-Hua MENG Lin JIANG Ling QIAN Guo-Zhen |
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| Abstract: | Objective: To observe the level of pro opiomelanocortin (POMC) mRNA expression in the hypothalamic arcuate nucleus in young and senile rats. Methods: POMC mRNA was determined with in situ hybridization, the hybridization signals and areas of POMC mRNA positive cells were measured with computer image analysis system. Results: Compared with young rats, average gray values of POMC mRNA positive neurons in the hypothalalmic arcuate nucleus and near region in the senile rats decreased, but the numbers of POMC mRNA positive neurons did not change significantly. Conclusion: The decrease of POMC mRNA expression level in individual neurons in hypothalamic arcuate nucleus of senile rats is one of the causes that decrease the expression of peptides derived from POMC gene. |
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| Keywords: | pro opiomelanocortin in situ hybridization image procession computer assisted |
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