| 丙型肝炎病毒NS3蛋白单克隆杂交瘤细胞株的建立及初步鉴定 |
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| 引用本文： | 高建恩,郭建平,纪和平,季颖,陶其敏.丙型肝炎病毒NS3蛋白单克隆杂交瘤细胞株的建立及初步鉴定[J].中华实验和临床病毒学杂志,1995(3). |
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| 作者姓名： | 高建恩 郭建平 纪和平 季颖 陶其敏 |
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| 作者单位： | 北京医科大学人民医院肝病研究所 |
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| 摘 要： | 应用国产基因工程表达的丙型肝炎病毒（ＨＣＶ）ＮＳ３区抗原免疫小鼠，然后取其脾细胞与小鼠骨髓瘤细胞系ＳＰ２／０融合，筛选出４株稳定分泌抗ＨＣＶＮＳ３区蛋白单克隆抗体（ＭｃＡｂ）的杂交瘤细胞株，分别命名为２Ｂ６，２Ｆ３，３Ｄ８，３Ｄ９，经初步研究表明这４株单抗与ＮＳ３抗原具有良好的反应性，与ＨＣＶ核心区多肽及乙型肝炎病毒表面抗原和ｅ抗原均无反应。抑制实验表明这４株抗体分别针对ＮＳ３抗原分子上的２个不同的抗原决定簇。
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| 关 键 词： | 抗体．单克隆，杂交瘤，肝炎病毒．丙型，细胞株 |
Establishment and Characterization of Hybridoma Cell Lines to Hepatitis C Virus NS3 protein |
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| Gao Jianen,Guo Jianping,Ji Heping,et al.Establishment and Characterization of Hybridoma Cell Lines to Hepatitis C Virus NS3 protein[J].Chinese Journal of Experimental and Clinical Virology,1995(3). |
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| Authors: | Gao Jianen Guo Jianping Ji Heping |
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| Abstract: | Four hybridoma cell lines secreting monoclonal antibodies against recombinant antigen of HCV NS3 region have been established. These strains are named as 2B6, 2F3,3D8 and 3D9, specifically reacting with HCV NS3 region protein but they have no reaction with synthesized or recombinant peptide of core region.The inhibition results indicate that 2B6 and 2F3.3D8 or 3D9 direct against two different epitopes of NS3 protein. |
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| Keywords: | Monoclonal antibody Hybridoma Hepatitis C virus Hepatitis C |
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