大肠癌细胞对5-氟尿嘧啶和奥沙利铂的敏感性

目的:比较5-氟尿嘧啶(5-FU)和亚叶酸(FA)联合应用(FUFA)、奥沙利铂(LOHP)以及LOHP加FUFA对DukesB和C期结直肠癌细胞的抑制作用.方法:收集术前未化疗,手术切除的大肠癌标本60例,将肿瘤标本制成浓度为2×105/ml细胞悬液,分别加入终浓度为0.01、0.1和1倍血浆峰值浓度(PPC)的FUFA、LO-HP和LOHP加FUFA,用MTT法测定肿瘤细胞生存率.结果:组间比较显示:FUFA和LOHP之间的综合疗效无统计学差异(P=0.338),FUFA和LOHP加FUFA之间也没有统计学差异(P=0.138).与LOHP单独用药相比较,LOHP、FUFA联合用药的综合肿瘤细胞存活率降低,显示出统计学上的差异(P=0.033).结论:对临床上确诊的DukesB和C期结直肠癌细胞,FUFA和LOHP在单药用药时都具有抑制作用,而该作用在LOHP加FUFA时增强,二药联合具有协同作用,这些作用呈现剂量相关性.

Drug Sensitivity of Colorectal Cancer Cells to 5-fluorouracil and Oxaliplatin

Objective: To compare the inhibitory effects of 5-fluorouracil(5-FU) combined with folinic acid(FA)(FUFA),oxaliplatin(LOHP) and combination of LOHP with FUFA on clinical diagnosed human colorectal cancer cells of Dukes stages B and C.Methods: Sixty operatively resected colorectal cancer tissue samples of patients without previous chemotherapy were collected.The tissues were processed to cancer cell suspension at concentration of 2×105/ml.Cells were exposed to FUFA,LOHP and LOHP+FUFA,and the final concentrations of the drugs were 0.01,0.1 and 1 time of peak plasma concentration(PPC).The survival rates of cancer cells were examined with MTT method.Results: There was no statistical difference between cancer cell survival rates(CCSR) in groups FUFA and LOHP(P=0.338),so was that between groups FUFA and LOHP+FUFA(P=0.138).The CCSR of group LOHP+FUFA was significant lower than that of group LOHP(P=0.033).Conclusions: Both FUFA and LOHP have inhibitory effect on clinical diagnosed Dukes B and C colorectal cancer cells.The effect is stronger when these drugs are combinatively used.A dose-related effect occurs.