链脲佐菌素诱导的糖尿病大鼠模型稳定性观察

目的:观察链脲佐菌素(STZ)诱导的1型糖尿病(TIDM)大鼠模型的稳定性,并探讨有关建模成功的标准.方法:模型组采用STZ按50mg/kg的剂量进行腹腔注射复制T1DM大鼠模型,对照组SD大鼠则腹腔注射等量的pH4.2、0.1mol/L柠檬酸-柠檬酸钠缓冲液,分别在腹腔注射后72h、1周、2周、3周、4周、5周、6周、7周、8周时检测各组大鼠的空腹血糖.结果:参照目前常用的腹腔注射STZ 72h后空腹血糖值≥16.7mmol/L即表示建模成功的标准,腹腔注射STZ后72h、1周、2周、3周、4周、5周、6周、7周、8周的建模成功率分别是100％、80％、75％、70％、70％、70％、70％、70％、70％.结论:如果采用目前常用的建模成功的标准,实际上在TIDM组中有30％的大鼠是未建模成功的,因此建议采用腹腔注射STZ后第3周空腹血糖值≥16.7mmol/L作为建模成功的标准.

The Stability of Type 1 Diabetes Rat Model Induced by STZ and Discussion on Standards of Successful Modeling

Objective: To observe the stability of type 1 diabetes rat model(T1DM) induced by streptozotocin(STZ),and to explore standards of successful modeling.Methods: T1DM model was established by intraperitoneal injection of STZ with a dose of 50 mg/kg,and rats in control group were given the same volume of pH 4.2,0.1 mol/L citric acid-citric sodium buffer solution instead.The fasting blood glucose of each group was detected respectively in 72 hours,1 week,2 weeks,3 weeks,4 weeks,5 weeks,6 weeks,7 weeks,and 8 weeks after injection of STZ.Results: According to common standards of successful model that the fasting blood glucose greater than or equal to 16.7 mmol/L after intraperitoneal injection of STZ for 72 h,the modeling successful rates of our T1DMs after injection for different time durations were 100%,80%,75%,70%,70%,70%,70%,70% and 70% respectively.Conclusions: Based on the current standards,30% T1DM model rats are failure actually.Fasting blood glucose ≥16.7 mmol/L kept for 3 weeks after injection of STZ as the standard for succesful modeling is proposed here.