Differential effects of N-methyl-d-aspartate on Ca homeostasis in developing and adult rat striatum: in vivo microdialysis approach |
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Authors: | Jerzy W ?azarewicz Ma?gorzata Puka-Sundvall Roman Gadamski Mats Sandberg Henrik Hagberg |
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Institution: | aMedical Research Centre, Polish Academy of Sciences, Dworkowa 3,00-784 Warsaw, Poland;bDepartment of Anatomy and Cell Biology, University of Göteborg, Medicinaregatan 3-5, S-413 90 Göteborg, Sweden |
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Abstract: | This in vivo study concerns developmental differences in the sensitivity of striatal neurons to N-methyl-d-aspartate (NMDA). Changes in calcium homeostatis in adult vs immature rats at postnatal days 8–10, evoked by NMDA, were evaluated by measurements of 45Ca efflux and of Ca2+ taurine and phosphoethanolamine concentrations in striatal microdialysates. The efflux of 14C]sucrose was employed in order to measure changes in extracellular space volume. In adult rats the addition of 5 mM NMDA for 20 min to the perfusion medium resulted in a 30–40% increase in 45Ca efflux, and in a 15% increase in 14C]sucrose efflux. Ten minutes after NMDA perfusion, 45Ca and 14C]sucrose efflux returned to the baseline. No significant changes in Ca2+ or amino acid concentrations were observed in the dialysate of the adult rat striatum. NMDA perfusion in the striatum of immature rats initially induced a transient (5 min) increase in the efflux of 45Ca (by 13%) and 14C]sucrose (by 9%). This was followed by a prolonged (lasting 45–50 min) 45% decrease in 45Ca efflux, an 80% reduction of Ca2+ concentration, and increases in taurine and phosphoethanolamine concentrations in the dialysate, whereas 14C]sucrose efflux recovered within 10 min.These data illustrate differences in the NMDA response between developing and adult rat striatum. Only in developing rats did NMDA induce a large and prolonged influx of extracellular calcium to neurons that may explain the enhanced NMDA neurotoxicity in immature rats. |
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Keywords: | striatal neurons NMDA 45C [14C]sucrose developing/adult rats |
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