肩髃穴电针对肩关节周围炎家兔模型的干预作用

目的]观察肩髃穴电针对肩关节周围炎家兔模型的干预作用,为该穴的作用机理提供实验依据。方法]将30只健康家兔(不分雌雄)完全随机分成三组,分别是模型对照组、肩髃穴电针组和布洛芬药物组每组10只,各组分别在造模前、治疗前、治疗后取兔耳动脉血以异构法测定血清PGE2含量,并且分别在治疗后切取兔患肩冈上肌腱、肱二头肌腱、冈下肌腱以样本碱水解法用生化仪在550nm处检测羟脯氨酸的含量。同时切取兔患肩局部组织,HE染色,在光镜下观测其组织形态的变化。结果]电针组和药物组血清中PGE2含量与模型组比较均无显著性差异,但肩髃穴电针治疗组治疗后血清中PGE2浓度低于治疗前,并有统计学意义。两治疗组肌腱中羟脯氨酸含量分别与模型对照组比较未有显著性差异,但布洛芬治疗组羟脯氨酸含量有高于电针治疗组的趋势。病理观察结果为电针肩髃穴可抑制炎症反应过强,抑制组织变性,促进肩周组织趋向好转。结论]电针肩髃穴具有抗炎镇痛作用;并可减低肩周组织机化程度,减慢其退行性改变。

Intervention of Electroacupuncture on Jianyu Acupoint to Rabbit Model with Scapulohumeral Periarthritis

Objective]To observe the intervention of Electroacupuncture at Point Jianyu(LI15) on Rabbit Model of Scapulohumeral Periarthritis for receiving possible mechanism.Methods ]Thirty healthy rabbits(male or female) were randomly divided into three groups:scapulohumeral periarthritis model group,EA Jianyu group and Ibuprofen control group.Each group respectively were detected the concentrations of serum PGE2 before model and treatment and after treatment,and then killed them to achieve tendons of shoulder which were detected the concentrations of Hydroxyproline.And achieve tissues around shoulder which were studied by HE to investigate the changes of the histology.Results]The concentrations of serum PGE2 had no notable difference between EA Jianyu and model group,Ibuprofen control group and model group after treatment.But it was lowered after treatment than before treatment by EA Jianyu.There were no significantly difference among the three groups about concentrations of Hydroxyproline in tendons,but Ibuprofen control group was higher than EA Jianyu group.Tissues of the shoulder were observed that EA Jianyu restrained reaction of inflammation and degenerescence of tissues,and promoted it better.Conclusions] EA Jianyu has anti-inflammatory effect and cuts back on the degree of degenerescence of tissues on scapulohumeral periarthritis model in rabbit.