洋紫荆中菲醌化合物bauhinione对K562细胞的增殖抑制作用

目的探讨从洋紫荆中分离得到的新的菲醌化合物bauhinione对体外培养的人类慢性骨髓性白血病K562细胞的增殖抑制作用。方法应用罗丹明B法检测bauhinione对癌细胞K562和tsFT210的抑制作用;采用形态学观察以及流式细胞术(FCM)检测该化合物对K562细胞的细胞周期抑制和诱导凋亡作用;通过倍量稀释法考察该化合物的最小抑制质量浓度及其质量浓度为12.5mg.L-1时的时效关系。结果Bauhinione对两种癌细胞的IC50值分别为(24.9±2.1)mg.L-1和(111.36±3.5)mg.L-1,说明K562细胞对bauhinione的作用敏感;Bauhinione对K562细胞的最小抑制质量浓度为6.25mg.L-1;对K562细胞的增殖抑制作用是通过G2-M期抑制和诱导凋亡实现的,并与时间呈依赖关系。结论Bauhinione在体外有抑制K562细胞增殖的作用,为洋紫荆的抗癌活性成分之一。

Cell proliferation inhibitory effect on human myeloid leukemia K562 of bauhinione from Bauhiniae variegata L.

Objective To investigate the cell proliferation inhibitory effect on human myeloid leukemia K562 of bauhinione,which obtained from the plant of Bauhiniae variegata L.Methods IC50 values were detected by SRB assay.The cell cycle inhibition and apoptosis inducing effect was analyzed by flow cytometry(FCM) accompanied with morphological observation under light microscope.The minimal inhibitory concentration(MIC) was assayed with the multiple dilution method.The time-effect relation at 12.5 mg·L-1 was detected.Results The IC50 value of bauhinione on the two cell lines were(24.9±2.1) mg·L-1 and(111.36±3.5) mg·L-1 respectively.The MIC value of bauhinione was 6.25 mg·L-1.The cell proliferation inhibitory effect of bauhinione was due to its G2-M phase inhibition and apoptosis induction.The time-effect relation curve was given.Conclusions Bauhinione has the cell proliferation inhibitory effect on K562 cell.