Foxp3+Tregs 在慢性间歇低氧所致肝损伤中的作用

摘要:目的 探讨叉头样转录因子 P3（Foxp3）阳性调节性 T 细胞（Tregs）在慢性间歇低氧诱导的肝损伤中的作用。方法 32 只雄性 Wistar 大鼠随机均分为空白对照组（A 组）、 高脂饮食组（B 组）、 间歇低氧组（C 组）和高脂饮食+间歇低氧组 （D 组）。实验暴露 4 周后留取血标本并分离出肝组织， 采用全自动分析仪检测各组大鼠血总胆固醇（TC）、 低密度脂蛋白胆固醇（LDL-C）、 丙氨酸转氨酶（ALT）、 天冬氨酸转氨酶（AST）的水平， 比色法测定肝组织丙二醛 （MDA） 含量， 放射免疫法检测促炎症细胞因子肿瘤坏死因子 （TNF） -α和白细胞介素 （IL） -1β水平， Western blot 法检测肝组织 Foxp3 蛋白表达。结果 各组 TC、 LDL-C 比较均是 B 组高于 A、 C、 D 组， D 组高于 A、 C 组 （均 P＜0.05），而 A 组与 C 组差异无统计学意义。ALT、 AST、 MDA、 TNF-α、 IL-1β水平比较均是 C 组高于 A 组， D 组高于 A、 B、 C 组（均 P＜0.05）， 而 A 组与 B 组、 B 组与 C 组间差异均无统计学意义。D 组肝组织 Foxp3 蛋白表达水平明显低于其他各组 （P＜0.05）。结论 Foxp3+Tregs 参与在高脂饮食基础上慢性间歇低氧所致的肝损伤的调节， 在此过程中可能起重要的保护性免疫作用。

The role of Foxp3+T cells in chronic intermittent hypoxia induced liver injury

Abstract: Objective To explore the role of Foxp3 + T cells (Tregs) in liver injury induced by chronic intermittent hypoxia. Methods Thirty-two male Wister rats were divided into four groups: control group (A), high-fat diet group (B), intermittent hypoxia group (C), and high- fat diet and intermittent hypoxia group (D). After 4 weeks, blood samples were collected and livers were surgically removed. Using the standard automatic clinical analyzer to test serum levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL- C), alanina aminotransferase (ALT) and aspartato aminotransferase (AST). The MDA content of liver tissue was measured by colorimetrc method. The levels of TNF-α and IL- 1β were measured by radiommunoassay, and the expression of Foxp3 protein was measured by Western blotting technique. Results Serum levels of TC and LDL-C were significantly higher in B group than those of A, C and D groups, and which were higher in D group than those of A and C groups (P＜0.05). There were no significant differences in serum levels of TC and LDL-C between A group and C group. Serum levels of ALT, AST, MDA, TNF-α and IL-1β were significantly higher in C group than those of A group, and which were significantly higher in D group than those of A, B and C groups (P＜0.05). There were no significant differences in these indicators between A group and B group, and between B group and C group. Foxp3 protein expression in liver was significantly lower in D group than that of other groups (P＜0.05). Conclusion Foxp3+ T regulatory cells involve in the regulation of hepatic injury induced by chronic intermittent hypoxia on the basis of a high-fat diet, and which may play an important role in this process of protective immune response.