唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究

目的:研究唾液乳酸杆菌w22a的潜在益生性和与种植体周围炎致病菌:牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、中间普氏菌(Pevotellaintermedia,Pi)、具核梭酸杆菌(Fusobacteriumncleatum,Fn)的体外作用,为乳酸杆菌w22a作为益生菌应用于种植体周围炎的防治提供实验依据。方法::通过检测w22a代谢产物成分、w22a对抑菌物质的耐受性等,初步探讨其口腔益生特性;并通过自聚和共聚实验、抑菌实验研究唾液乳酸杆菌w22a和种植体周围炎致病菌Pg、Pi、Fn的体外作用。结果:发现唾液乳酸杆菌w22a可产生有机酸、过氧化氢等抑菌物质,并能产生胞外多糖;w22a能够耐受一定浓度的抗生素和溶菌酶;w22a在体外有自聚集和与Pg、Pi、Fn发生共聚集的能力;其代谢产物对于Pg、Pi、Fn的生长具有不同程度的抑制作用。结论:唾液乳酸杆菌w22a能够对Pg、Pi、Fn产生生长抑制作用,具有口腔益生性,有望作为益生菌用于种植体周围炎的防治。

Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.

Objective: To evaluate whether the oral administration of Lactobacillus salivarius w22a could alleviate the peri-implantitis pathogens in subgingival plaque, such as Porphyromonas gingivalis (Pg), Pevotella intermedia (Pi), and Fusobacteriurn nuclearum (Fn) in vitro.Methods: The antagonistic growth effects of Lactobacillus salivarius as well as their probiotic on periodontal pathogens, including Pg, Pi and Fn, were tested using drug resistance experiment, agglutination experiments, and antibacterial experiments.Results: The results revealed that Lactobacillus salivarius w22a could produce antibiotics such as organic acid, hydrogen peroxide and EPS. Strain w22a could resist lysozyme and antibiotics at certain concentration. In addition, auto-agglutination and agglutination with other pathogens could be observed. The fermentation of w22a inhibited the growth of pathogens to varied degrees.Conclusion: Lactobacillus salivarius w22a was a potential probiotic strain to inhibit the growth of Pg, Pi and Fn in vitro.