纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备

目的:在纳米羟基磷灰石(nHA)涂层表面制备稳定可重复的阿伦膦酸钠(ALN)和I型胶原蛋白(COL I)复合膜。方法:在纯钛(PT)表面电化学沉积nHA涂层,以3-氨丙基三乙氧基硅烷(APTES)、1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)为偶联剂,在涂层表面结合ALN和COL I,制备nHA/ALN/COL I和nHA/COL I/ALN复合膜。通过扫描电子显微镜(SEM),X射线能谱分析仪(EDS)和X射线衍射仪(XRD)表征nHA涂层,通过X射线光电子能谱仪(XPS)和傅里叶红外光谱仪(FTIR)表征复合膜。结果:ALN和COL I可通过偶联剂结合于nHA涂层,改变其结合次序,可分别形成nHA/ALN/COL I和nHA/COL I/ALN复合膜。结论:通过化学结合法形成的nHA/ALN/COL I和nHA/COL I/ALN复合膜具有不同的ALN缓释潜力。

Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface.

Objective: To form a stable and repeatable alendronate sodium (ALN) and collagen type I (COL I) complex films on nano-hydroxyapatite (nHA) layer. Methods: A nHA layer was coated on the pure titanium (PT) surface by using electrochemical deposition, then ALN and COL I were chemically bonded on the nHA layer to form nHA/ALN/COL I and nHA/COL I/ALN complex films using 3-aminopropyl triethoxysilane (APTES) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) as coupling agent. The nHA layer was characterized by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffractometer (XRD), and the complex films were characterized by X-ray photoelectron spectrometer (XPS) and Fourier transform infrared spectroscopy (FTIR). Results: ALN and COL I were bonded on the nHA layer by coupling agent, nHA/ALN/COL I and nHA/COL I/ALN complex films were formed by changing the binding order of ALN and COL I. Conclusion: The nHA/ALN/COL I and nHA/COL I/ALN complex films formed by chemical covalent bonding method have different potentials of ALN sustained-release.